Neuropathic pain caused by lesions or nervous system dysfunction is a neuroimmune disease with limited therapeutic options. Adipose tissue-derived stem cells (ASCs) are multipotent mesenchymal stem cells with potent immunosuppressive properties, and their use as novel cell-based therapeutics have been proposed in many immune diseases. However, the analgesic effect and efficacy of ASCs to treat neuropathic pain remain unclear. This study, thus, investigated whether ASCs or ASC-derived culture medium can relieve neuropathic pain behaviors (i.e., mechanical and cold allodynia) in a rat model with L5 spinal nerve ligation. Intrathecal injection of ASCs significantly reduced cold allodynia, but not mechanical allodynia. Importantly, cold allodynia was completely reversed in rats with repeated injections of ASCs. In contrast, intrathecal injection of ASC-derived culture medium or retro-orbital injection of ASCs had no effect on neuropathic pain behaviors. These results suggest a novel and alternative therapeutic application of ASCs to target specific neuropathic pain behaviors.

中文翻译：

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脂肪组织衍生的干细胞缓解神经性疼痛大鼠脊髓神经结扎模型中的冷异常性疼痛

由病变或神经系统功能障碍引起的神经性疼痛是一种神经免疫疾病，治疗选择有限。脂肪组织干细胞（ASCs）是具有有效免疫抑制特性的多能间充质干细胞，在许多免疫疾病中已提出将其用作新型基于细胞的疗法。但是，ASC的镇痛效果和治疗神经性疼痛的疗效仍不清楚。因此，这项研究调查了ASC或ASC衍生的培养基是否可以减轻L5脊髓神经结扎大鼠模型的神经性疼痛行为（即机械性和冷性异常性疼痛）。鞘内注射ASC可以显着降低感冒异常性疼痛，但不能减少机械性异常性疼痛。重要的是，反复注射ASC可使大鼠的冷异常性疼痛完全逆转。相反，复古的ASC -orbital注射对神经性疼痛的行为没有任何影响。这些结果表明ASC靶向特定的神经性疼痛行为的新颖和替代治疗应用。