Increasing the migratory capacity of the implanted mesenchymal stem cells (MSCs) is a major challenge in developing successful cell transplantation therapies. Nevertheless, the regulatory factors involved in the migration of BMMSCs remain largely unknown. In this study, we studied the role of the peripherin (PRPH) gene in regulating the ability of Wuzhishan mini pig (WZSP) BMMSCs to migrate in vitro. Four different shRNA vectors directed against PRPH were designed and transfected into BMMSCs. The vector with the best interference effect was chosen to be used in the following experiments. The expression level of PRPH in BMMSCs was determined by quantitative real-time PCR and western blot analysis. The migration capacity of the BMMSCs was estimated using a scratch assay, a transwell in vitro migration model assay, and filamentous actin staining. The results showed that shRNA-mediated knockdown of the expression of the PRPH gene in BMMSCs reduced the ability of these cells to migrate. Overall, these results illustrate that the PRPH gene regulates the migration of BMMSCs in the WZSP.

中文翻译：

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Peripherin基因调节五指山小型猪骨髓间充质干细胞的迁移

提高植入的间充质干细胞（MSC）的迁移能力是开发成功的细胞移植疗法的主要挑战。然而，BMMSC迁移所涉及的调控因素仍然未知。在这项研究中，我们研究了外周蛋白（PRPH）基因在调节五指山小型猪（WZSP）BMMSC体外迁移能力中的作用。设计了四种针对PRPH的不同shRNA载体，并将其转染到BMMSC中。选择具有最佳干扰效果的载体以用于以下实验。通过定量实时PCR和蛋白质印迹分析确定PRMSC在BMMSC中的表达水平。BMMSC的迁移能力使用刮擦测定法，Transwell体外迁移模型测定法和丝状肌动蛋白染色来估计。结果表明，shRNA介导的BMMSC中PRPH基因表达的敲低降低了这些细胞迁移的能力。总体而言，这些结果说明PRPH基因调控WZSP中BMMSC的迁移。