Mediterranean plant Helichrysum italicum represents a rich source of versatile bioactive compounds with potential benefits for human health. Despite extensive research on the plant’s active constituents, little attention has yet been paid to characterizing the relationship between its intra-specific genetic diversity and metabolite profile. The study aimed to determine metabolic profile of H. italicum ssp. italicum (HII) and ssp. tyrrhenicum (HIT) cultivated on the experimental plantation in Slovenia and to compare the chemical composition of extracts regarding the solvent extraction process. Extracts were prepared upon conventional extract preparation procedures: maceration with 50 % methanol or ethanol and cold or hot water infusion and analyzed using High Performance Liquid Chromatography-Diode Array Detection-Electrospray Ionization-Quadrupole Time-of-Flight-Mass Spectrometry (HPLC-DAD-ESI-QTOF-MS). One hundred compounds were identified in the samples, among them several isomers and derivatives were reported for the first time, while caffeoylquinic acids and pyrones were the most abundant. Semi-quantitative comparison revealed that the extraction procedure had a greater impact on the chemical profile than genetic variability. All HIT extracts showed a higher total phenolic content compared to HII, while the antioxidant potential evaluated by 1,1-diphenyl-2-picrylhydrazil test was not proportionally higher. In addition, hot water extracts proved to be comparably active as alcoholic ones, confirming high commercial potential of Helichrysum italicum as herbal functional beverages.

中文翻译：

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HPLC-DAD-ESI-QTOF-MS 测定两种永久花的水醇和水提取物的生物活性化合物和抗氧化活性比较


地中海植物意大利蜡菊是多种生物活性化合物的丰富来源，对人类健康具有潜在益处。尽管对植物的活性成分进行了广泛的研究，但很少有人关注其种内遗传多样性和代谢谱之间的关系。该研究旨在确定H. italicum ssp 的代谢特征。 italicum ( HII ) 和 ssp。在斯洛文尼亚的实验种植园中种植的Tyrhenicum ( HIT )，并比较溶剂提取过程中提取物的化学成分。提取物按照常规提取物制备程序制备：用 50% 甲醇或乙醇浸渍并注入冷水或热水，并使用高效液相色谱-二极管阵列检测-电喷雾电离-四极杆飞行时间质谱 (HPLC-DAD) 进行分析-ESI-QTOF-MS)。样品中鉴定出100种化合物，其中多种异构体和衍生物为首次报道，其中咖啡酰奎尼酸和吡喃酮含量最丰富。半定量比较表明，提取过程对化学特征的影响比遗传变异性更大。与HII相比，所有HIT提取物均显示出更高的总酚含量，而通过 1,1-二苯基-2-苦基肼测试评估的抗氧化潜力并未成比例地更高。此外，热水提取物被证明具有与酒精提取物相当的活性，证实了意大利蜡菊作为草本功能饮料的巨大商业潜力。