Freshwater Synechocystis sp. PCC 6803 has been considered to be a platform for the production of the next generation of biofuels and is used as a model organism in various fields. Various genomics, transcriptomics, metabolomics, and proteomics studies have been performed on this strain, whereas marine Synechocystis sp. PCC 7338 has not been widely studied despite its wide distribution. This study analyzed the proteome profiles of two Synechocystis strains using a liquid chromatography–tandem mass spectrometry-based bottom-up proteomic approach. Proteomic profiling of Synechocystis sp. PCC 7338 was performed for the first time with a data-dependent acquisition method, revealing 18,779 unique peptides and 1794 protein groups. A data-independent acquisition method was carried out for the comparative quantitation of Synechocystis sp. PCC 6803 and 7338. Among 2049 quantified proteins, 185 up- and 211 down-regulated proteins were defined in Synechocystis sp. PCC 7338. Some characteristics in the proteome of Synechocystis sp. PCC 7338 were revealed, such as its adaptation to living conditions, including the down-regulation of some photosynthesis proteins, the up-regulation of kdpB, and the use of osmolyte glycine as a substrate in C1 metabolism for the regulation of carbon flow. This study will facilitate further studies on Synechocystis 7338 to define in depth the proteomic differences between it and other Synechocystis strains.

中文翻译：

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使用液相色谱-串联质谱法对海洋和淡水拟蓝藻菌株进行蛋白质组学比较分析

淡水集胞藻 PCC 6803被认为是生产下一代生物燃料的平台，并在各个领域用作模型生物。已经对该菌株进行了各种基因组学，转录组学，代谢组学和蛋白质组学研究，而海洋集胞藻属（Synechocystis sp。）尽管PCC 7338分布广泛，但尚未得到广泛研究。这项研究使用基于液相色谱-串联质谱的自下而上的蛋白质组学方法分析了两个集胞藻菌株的蛋白质组谱。变形囊藻的蛋白质组学分析sp。PCC 7338首次使用了依赖数据的采集方法，揭示了18779个独特的肽和1794个蛋白质组。进行了一种独立于数据的采集方法，用于对蓝藻属藻进行比较定量。PCC 6803和7338。在2049个定量蛋白中，Synechocystis sp。中定义了185个上调和211个下调的蛋白。PCC 7338.在蛋白质组一些特征集胞藻。揭示了PCC 7338，例如其对生活条件的适应性，包括某些光合作用蛋白的下调，kdpB的上调，以及渗透压甘氨酸作为C1代谢中底物的碳流量调节。这项研究将有助于进一步研究蓝藻7338深入定义了它与其他蓝藻菌株之间的蛋白质组差异。