ABSTRACT

Transcriptional repressor GATA binding 1 (TRPS1) is a newly discovered transcription factor, which has been reported in many tumors, except for gastric cancer (GC). In this study, we aimed to grope for clinical significance and biological function of TRPS1 in GC. TRPS1 expression in GC and its relationship with clinicopathological features were analyzed based on public databases, and verified by immunohistochemistry and RT-qPCR. Kaplan-Meier survival curve and Cox regression model were used to estimate the influence of TRPS1 on the univariate prognosis and multivariate survival risk factors of GC. The effects of TRPS1 on malignant biological behaviors of GC cells were studied by CCK8 cell proliferation, scratch test, and Transwell assay. The function of TRPS1 was further analyzed by signaling pathway analysis. TRPS1 mRNA expression in GC tissues was up-regulated and was of great significance in some prognostic factors. Protein expression of TRPS1 in tumor tissues was significantly higher than that in paracancerous tissues. Over-expression of TRPS1 was a poor prognostic indicator for GC patients. TRPS1 knockdown could inhibit the proliferation, migration, and invasion of GC cells. The important role of TRPS1 was in the extracellular matrix, and it was involved in actin binding and proteoglycan in cancer. The hub genes of TRPS1 (FN1, ITGB1) were defined. TRPS1 may be a tumor promoter and promote the development of GC by influencing the malignant biological behaviors of GC. TRPS1 is expected to be a key diagnostic and prognostic indicator for GC patients.

摘要

转录抑制因子 GATA 结合 1 (TRPS1) 是一种新发现的转录因子，在除胃癌 (GC) 之外的许多肿瘤中均有报道。在本研究中，我们旨在探索 TRPS1 在 GC 中的临床意义和生物学功能。基于公共数据库分析GC中TRPS1表达及其与临床病理特征的关系，并通过免疫组织化学和RT-qPCR验证。Kaplan-Meier生存曲线和Cox回归模型用于估计TRPS1对GC单因素预后和多因素生存危险因素的影响。通过CCK8细胞增殖、划痕试验和Transwell实验研究TRPS1对GC细胞恶性生物学行为的影响。通过信号通路分析进一步分析了TRPS1的功能。TRPS1GC组织中mRNA表达上调，在某些预后因素中具有重要意义。TRPS1在肿瘤组织中的蛋白表达明显高于癌旁组织。TRPS1 的过度表达是 GC 患者的不良预后指标。TRPS1敲低可以抑制GC细胞的增殖、迁移和侵袭。TRPS1在细胞外基质中的重要作用是参与癌症中的肌动蛋白结合和蛋白多糖。定义了TRPS1 (FN1, ITGB1)的中枢基因。TRPS1可能是肿瘤启动子，通过影响GC的恶性生物学行为促进GC的发生发展。TRPS1 有望成为 GC 患者的关键诊断和预后指标。