ABSTRACT

Skeletal muscle, a critical component of the mammalian body, is essential for normal body movement. miRNAs are well documented in gene post-transcription regulation in many biological processes, including muscle development and maintenance. miR-92b-3p, which is often associated with tumorigenesis, has never been explored in myoblast development. Here, we used murine-derived C2C12 myoblasts to explore the potential functions of miR-92b-3p in skeletal muscle development. Our results demonstrated that miR-92b-3p mimics inhibited C2C12 cell proliferation and migration, whereas miR-92b-3p inhibitor promoted C2C12 cell proliferation and migration. C2C12 cell differentiation was not affected by miR-92b-3p mimics, according to immunofluorescence and qPCR results. Serum- and glucocorticoid-induced kinase 3 (SGK3) was predicted and validated as a target of miR-92b-3p. Overexpression of SGK3 promoted C2C12 cell proliferation. SGK3 and miR-92b-3p formed a regulatory pathway to modulate C2C12 cell proliferation. In conclusion, miR-92b-3p inhibited C2C12 cell proliferation by targeting SGK3 and impeded C2C12 cell migration.

摘要

骨骼肌是哺乳动物身体的重要组成部分，对于正常的身体运动至关重要。miRNA 在许多生物过程（包括肌肉发育和维持）中的基因转录后调控中得到充分证明。通常与肿瘤发生相关的 miR-92b-3p 从未在成肌细胞发育中被探索过。在这里，我们使用鼠源性 C2C12 成肌细胞来探索 miR-92b-3p 在骨骼肌发育中的潜在功能。我们的结果表明 miR-92b-3p 模拟物抑制 C2C12 细胞增殖和迁移，而 miR-92b-3p 抑制剂促进 C2C12 细胞增殖和迁移。根据免疫荧光和 qPCR 结果，C2C12 细胞分化不受 miR-92b-3p 模拟物的影响。血清和糖皮质激素诱导的激酶 3 (SGK3) 被预测并验证为 miR-92b-3p 的靶标。SGK3 的过表达促进 C2C12 细胞增殖。SGK3 和 miR-92b-3p 形成调节 C2C12 细胞增殖的调节途径。总之，miR-92b-3p通过靶向SGK3抑制C2C12细胞增殖并阻碍C2C12细胞迁移。