ABSTRACT

In this paper, laccase is immobilized by the cross-linking method, using organic fertilizer as a carrier and glutaraldehyde as a cross-linking agent. Here, the optimal conditions of laccase immobilization were explored and the optimal operating conditions and stabilities of free laccase and immobilized laccase were also studied. Then, free laccase and immobilized laccase were applied to the soil remediation. Meanwhile, the effect of soil improvement treated with immobilized laccase was studied through ecological evaluation. The results showed that the optimal conditions for laccase immobilization were: the volume fraction of glutaraldehyde was 5%, the amount of enzyme added was 15 mL, and the immobilization time was 6 h. Under the same conditions, thermal stability and acid-base stability of immobilized laccase were better than free laccase. Under the optimal conditions, using laccase to treat 2,4-dichlorophenol in the soil, it was found that the free laccase group degraded 44.4% within 5 days, while the immobilized laccase group degraded 58.6%. Although both the degradation trends and route are the same, the degradation effect of the latter is obviously better. Ecological evaluation showed that organic fertilizer carrier had an impact on soil physical and chemical properties and soil enzymes, playing a positive role in soil ecological security and improving the soil.

摘要

本文以有机肥料为载体，戊二醛为交联剂，通过交联法固定漆酶。在此，探讨了漆酶固定化的最佳条件，并研究了游离漆酶和固定化漆酶的最佳操作条件和稳定性。然后，将游离漆酶和固定化漆酶应用于土壤修复。同时，通过生态评价研究了固定化漆酶处理土壤改良的效果。结果表明，漆酶固定化的最佳条件为：戊二醛的体积分数为5％，酶的加入量为15 mL，固定时间为6 h。在相同条件下，固定化漆酶的热稳定性和酸碱稳定性均优于游离漆酶。在最佳条件下，用漆酶处理土壤中的2，4-二氯苯酚，发现游离漆酶基团在5天内降解了44.4％，而固定化漆酶基团降解了58.6％。尽管降解趋势和途径相同，但后者的降解效果明显更好。生态评价表明，有机肥载体对土壤理化性质和土壤酶的影响，对土壤生态安全和改善土壤有积极作用。