ABSTRACT The plant extracellular vesicles (EVs) are lipid-enveloped nano-particles containing proteins, nucleic acids and metabolites and function in plant development and response. The Arabidopsis four transmembrane protein TETRASPANIN 8 (TET8) knock-out mutant tet8 secreted less EVs than the wild-type (WT). In this report, we show that the tet8 mutant was attenuated in the plant hormone jasmonate (JA) accumulation in response to mechanical wounding treatment. We also noticed that the EVs contained a high level of phospholipids phosphatidic acids (PAs) which may serve as precursors of JA biosynthesis during wound-triggered-self-healing processes. Thus, we propose an open question about a potential role of EVs or TET8 or both in damage-associated JA response.

中文翻译：

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拟南芥叶细胞外囊泡在伤口诱导茉莉酸积累中的作用

摘要 植物细胞外囊泡 (EVs) 是脂质包裹的纳米颗粒，含有蛋白质、核酸和代谢物，在植物发育和反应中发挥作用。拟南芥四跨膜蛋白 TETRASPANIN 8 (TET8) 敲除突变体 tet8 比野生型 (WT) 分泌更少的 EV。在本报告中，我们显示 tet8 突变体在植物激素茉莉酮酸 (JA) 积累中减弱，以响应机械创伤处理。我们还注意到 EVs 含有高水平的磷脂磷脂酸 (PAs)，它可以作为伤口触发自愈过程中 JA 生物合成的前体。因此，我们提出了一个悬而未决的问题，即 EV 或 TET8 或两者在损伤相关 JA 反应中的潜在作用。