Abstract

The dried root of Astragalus membranaceus is a well-known herbal medicine, and it is useful in treating chronic diseases and weakness, as well as for improving overall health and vitality. Astragalosides, which are root quality indicators of A. membranaceus, are natural triterpenoid saponins that are used in the treatment of diabetes and cardiovascular diseases. Currently, there is an urgent need to improve their production because of their low quantity in plants and the difficulty of chemical synthesis. In this study, yeast extract was added to facilitate elicitation in Agrobacterium-mediated hairy root cultures, thereby enhancing astragaloside production in A. membranaceus. Results showed that yeast extract could stimulate astragaloside content effectively in the hairy roots of A. membranaceus. Moreover, astragaloside accumulation was positively correlated with the upregulation of mevalonate biosynthetic gene expression in the presence of yeast extract. Our study demonstrated that pretreatment with yeast extract (3.65 mM) for 72 h serves as an effective strategy to enhance astragaloside levels in A. membranaceus hairy root cultures. Thus, these optimal conditions can provide valuable information for the improvement of astragaloside industrial production in A. membranaceus.

摘要

黄芪的干根是一种著名的草药，可用于治疗慢性疾病和虚弱，以及改善整体健康和活力。黄芪皂苷是A. membranaceus 的根质量指标，是用于治疗糖尿病和心血管疾病的天然三萜皂苷。目前，由于它们在植物中的数量少，化学合成困难，迫切需要提高它们的产量。在这项研究中，添加酵母提取物以促进农杆菌介导的毛状根培养物中的诱导，从而提高A. membranaceus 中黄芪甲苷的产量. 结果表明，酵母抽提物能有效提高膜松毛根中黄芪甲苷的含量。此外，在酵母提取物存在下，黄芪甲苷的积累与甲羟戊酸生物合成基因表达的上调呈正相关。我们的研究表明，用酵母提取物 (3.65 mM) 预处理 72 小时是提高A. membranaceus毛状根培养物中黄芪甲苷水平的有效策略。因此，这些最佳条件可以为黄芪甲苷工业生产的改善有价值的信息黄芪。