Abstract

In this work, three fungal endophytes identified as Aspergillus terreus (AFL, AFSt and AFR), were studied for their antioxidant potential. LC-MS-based metabolomics, followed by multivariate statistical analysis were then applied to comprehensively profile their extracts. The three fungal endophytes revealed interesting antioxidant potential, in particular, the strain isolated from the Artemisia annua leaves (AFL), which was rich in different types of phenolic metabolites. Additionally, all fungal-derived ethyl acetate extracts showed potent inhibition against the prooxidant xanthine oxidase. Multivariate analysis (PCA and PLS-DA) demonstrated a unique chemical fingerprint for each strain, where phenolics, coumarins, and polyketides were the discriminative metabolites of the three fungal strains. The present findings highlighted the power of metabolomics in the chemotaxonomical classification of closely related strains. It also asserted the role of fungal endophytes in the management of oxidative stress, particularly when they are utilized in the production of fermented food products.

摘要

在这项工作中，研究了三种被鉴定为土曲霉（AFL、AFSt 和 AFR）的真菌内生菌的抗氧化潜力。然后应用基于 LC-MS 的代谢组学和多变量统计分析来全面分析其提取物。三种真菌内生菌显示出有趣的抗氧化潜力，特别是从青蒿中分离出的菌株叶（AFL）富含不同类型的酚类代谢物。此外，所有真菌衍生的乙酸乙酯提取物都显示出对促氧化剂黄嘌呤氧化酶的有效抑制作用。多变量分析（PCA 和 PLS-DA）证明了每种菌株的独特化学指纹，其中酚类、香豆素和聚酮化合物是三种真菌菌株的区分代谢物。目前的研究结果强调了代谢组学在密切相关菌株的化学分类学分类中的作用。它还断言了真菌内生菌在氧化应激管理中的作用，特别是当它们被用于生产发酵食品时。