ABSTRACT

Periodontitis is a bacterial biofilm-induced oral disease, mostly caused by Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis). Oral administration of chicken egg yolk antibody (IgY) is a promising nutritional strategy to control pathogen infections. The objective of this study was to produce an A. actinomycetemcomitans- and P. gingivalis-specific IgY and evaluate its effects on bacterial agglutination and biofilm formation. Thirty laying hens were immunized with a complex of lysate containing typical molecular weights of membrane proteins of A. actinomycetemcomitans and P. gingivalis. IgY was isolated by polyethylene glycol 6000 and ammonium sulfate and purified by dialysis. The results of enzyme-linked immunosorbent assay showed that the obtained IgY were specific to both A. actinomycetemcomitans and P. gingivalis. In addition, immunoelectron microscopy scanning and crystal violet staining showed that the IgY could bind to cell wall of the pathogens and efficiently accelerate agglutination and inhibit biofilm formation. Furthermore, the activity of the IgY remained stable at different temperature, pH, and storage period. This is the first report that a novel two-in-one IgY was produced to modulate the agglutination and biofilm formation of A. actinomycetemcomitans and P. gingivalis, suggesting the potential of IgY to control periodontitis caused by oral pathogens.

摘要

牙周炎是一种由生物膜引起的细菌性口腔疾病，主要由聚集性放线杆菌 （A. actinomycetemcomitans）和 牙龈卟啉单胞菌 （P. gingivalis）引起。口服蛋黄抗体（IgY）是控制病原体感染的一种有前景的营养策略。这项研究的目的是生产 放线放线菌和 牙龈卟啉单胞菌特异性的IgY，并评估其对细菌凝集和生物膜形成的影响。用含有典型分子量的放线杆菌 和 牙龈卟啉单胞菌膜蛋白分子量的裂解物复合物免疫30只蛋鸡 。。通过聚乙二醇6000和硫酸铵分离IgY，并通过透析纯化。酶联免疫吸附试验的结果表明，获得的IgY对放线杆菌 和 牙龈卟啉单胞菌均具有特异性 。此外，免疫电子显微镜扫描和结晶紫染色表明，IgY可以结合到病原体的细胞壁上，并有效地加速凝集并抑制生物膜的形成。此外，IgY的活性在不同的温度，pH和储存时间下保持稳定。这是第一个报道了新型二合一IgY的产物，可调节A.放线菌 和 牙龈卟啉单胞菌的凝集和生物膜形成 。，提示IgY控制口腔病原体引起的牙周炎的潜力。