Abstract In this second research report (i.e., Part II), an ionic liquid (IL)-bonded silica column having an anion-exchange characteristic is described and characterized chromatographically with test acidic solute probes and by physical measurements such as Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA). The IL anion-exchange stationary phase consists of a short alkyl chain IL stationary phase that has been synthesized by covalently immobilizing 1-ethylimidazole on a silica substrate through chloropropyltrimethoxysilane tether. The retention characteristics of the resulting stationary phase have been examined with acidic solutes such as aromatic acid derivatives and nucleotides. The test results show good resolution and separation efficiency indicating the presence of different interaction mechanisms such as π–π, electrostatic and hydrophobic interactions to a certain extent. Abbreviations: ABs: alkylbenzenes; ADP: adenosine 5′-diphosphate; AMP: adenosine 5′-monophosphate; CPTMS: 3-chloropropyltrimethoxysilane; ILs: ionic liquids; UMP: uridine 5′-monophosphate; UDP: uridine 5′-diphosphate; UTP: uridine 5′-triphosphate. GRAPHICAL ABSTRACT

中文翻译：

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咪唑鎓离子液体键合硅胶固定相。第二部分。1-乙基咪唑鎓固定相

摘要 在第二份研究报告（即第二部分）中，描述了一种具有阴离子交换特性的离子液体 (IL) 键合硅胶色谱柱，并使用测试酸性溶质探针进行色谱分析，并通过傅里叶变换红外 (FTIR) 等物理测量进行了表征。 ) 和热重分析 (TGA)。IL 阴离子交换固定相由短烷基链 IL 固定相组成，该固定相是通过氯丙基三甲氧基硅烷系链将 1-乙基咪唑共价固定在硅胶基质上合成的。所得固定相的保留特性已用酸性溶质（如芳香酸衍生物和核苷酸）进行了检查。测试结果显示出良好的分辨率和分离效率，表明存在不同的相互作用机制，如 π–π、静电和疏水相互作用在一定程度上。缩写： ABs：烷基苯；ADP：5'-二磷酸腺苷；AMP：5'-单磷酸腺苷；CPTMS：3-氯丙基三甲氧基硅烷；ILs：离子液体；UMP：尿苷 5'-单磷酸；UDP：尿苷 5'-二磷酸；UTP：尿苷 5'-三磷酸。图形概要