Rice cultivars from japonica and indica lineage possess differential resistance against blast fungus as a result of genetic divergence. Whether different rice cultivars also show distinct metabolomic changes in response to P. oryzae, and their role in host resistance, are poorly understood. Here, we examine the responses of six different rice cultivars from japonica and indica lineage challenged with P. oryzae. Both susceptible and resistant rice cultivars expressed several metabolites exclusively during P. oryzae infection, including the saponin Bayogenin 3‐O‐cellobioside. Bayogenin 3‐O‐cellobioside level in infected rice directly correlated with their resistant attributes. These findings reveal, for the first time to our knowledge that besides oat, other grass plants including rice produces protective saponins. Our study provides insight into the role of pathogen‐mediated metabolomics reprogramming in host immunity. The correlation between Bayogenin 3‐O‐Cellobioside levels and blast resistance suggests that engineering saponin expression in cereal crops represents attractive and sustainable disease management.

中文翻译：

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Bayogenin 3-O-纤维二糖苷赋予对稻瘟病菌 Pyrularia oryzae 的非品种特异性防御


由于遗传差异，粳稻和籼稻谱系的水稻品种对稻瘟病菌具有不同的抗性。不同的水稻品种是否也表现出针对米曲霉的不同代谢组变化，以及它们在宿主抗性中的作用，人们知之甚少。在这里，我们研究了来自粳稻和籼稻谱系的六种不同水稻品种对米假单胞菌攻击的反应。敏感和抗性水稻品种在米假单胞菌感染期间只表达几种代谢物，包括皂苷Bayogenin 3-O-纤维二糖苷。受感染水稻中的Bayogenin 3-O-纤维二糖苷水平与其抗性特性直接相关。据我们所知，这些发现首次表明，除了燕麦之外，包括水稻在内的其他草类植物也能产生保护性皂苷。我们的研究深入了解了病原体介导的代谢组学重编程在宿主免疫中的作用。 Bayogenin 3-O-纤维二糖苷水平与稻瘟病抗性之间的相关性表明，在谷类作物中改造皂苷表达代表着有吸引力且可持续的疾病管理。