Perfluorooctanesulfonic acid (PFOS) is a perfluorinated compound used in many industrial and consumer products. It has been linked to a broad range of adverse effects in several species, including zebrafish (Danio rerio). The zebrafish embryo is a widely used vertebrate model to elucidate potential adverse effects of chemicals because it is amenable to medium and high throughput. However, there is limited research on the full extent of the impact the chorion has on those effects. Results from the present study indicate that the presence of the chorion affected the timing and incidence of mortality as well as morphometric endpoints such as spinal curvature and swim bladder inflation in zebrafish embryos exposed to PFOS. Furthermore, removal of the chorion prior to exposure resulted in a lower threshold of sensitivity to PFOS for effects on transcriptional expression within the peroxisome proliferator–activated receptor (PPAR) nuclear signaling pathway. Perturbation of PPAR pathway gene expression can result in disruption of metabolic signaling and regulation, which can adversely affect development, energy availability, and survival. It can be concluded that removal of the chorion has significant effects on the timing and incidence of impacts associated with PFOS exposure, and more research is warranted to fully elucidate the protective role of the chorion and the critical timing of these events. Environ Toxicol Chem 2021;40:780–791. Published 2020. This article is a US Government work and is in the public domain in the USA. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

中文翻译：

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在存在或不存在绒毛膜的情况下全氟辛烷磺酸对斑马鱼胚胎的毒性

全氟辛烷磺酸 (PFOS) 是一种全氟化化合物，用于许多工业和消费品。它与包括斑马鱼（ Danio rerio ）在内的多个物种的广泛不利影响有关。斑马鱼胚胎是一种广泛使用的脊椎动物模型，用于阐明化学品的潜在不利影响，因为它适合中高通量。然而，关于绒毛膜对这些影响的全部影响程度的研究有限。本研究的结果表明，绒毛膜的存在会影响死亡的时间和发生率，以及暴露于全氟辛烷磺酸的斑马鱼胚胎的形态学终点，例如脊柱弯曲和鱼鳔膨胀。此外，在暴露之前去除绒毛膜会导致对 PFOS 的敏感性阈值降低，从而影响过氧化物酶体增殖物激活受体 (PPAR) 核信号通路内的转录表达。PPAR 通路基因表达的扰动可能导致代谢信号和调节的破坏，从而对发育、能量可用性和生存产生不利影响。可以得出结论，去除绒毛膜对与 PFOS 暴露相关的影响的时间和发生率具有显着影响，需要进行更多研究来充分阐明绒毛膜的保护作用以及这些事件的关键时间。环境毒理学化学2021；40：780–791。2020 年出版。本文是美国政府的作品，在美国属于公共领域。《环境毒理学和化学》由 Wiley periodicals LLC 代表 SETAC 出版。