Rabies is a devastating disease affecting almost all mammalian animal species including humans. Vaccines are available to combat the disease. Protection against the disease is rendered by assessing the humoral immune response. Recent reports suggest the role of cell mediated immune response (CMI) in assessing vaccine efficacy. In the present study, two live vectored vaccine candidates containing glycoprotein G of rabies virus were generated using the mesogenic Newcastle disease virus (NDV) strain R2B and another with NDV with an altered fusion protein cleavage site as backbones. The efficacy of these vaccine candidates on testing in experimental mouse model indicated generation of robust humoral and CMI responses. The recombinant NDV containing the altered fusion protein cleavage site with glycoprotein G showed the highest CMI response in mice indicating its usage as a potential live vectored vaccine candidate against the disease.

狂犬病是一种破坏性疾病，影响到几乎所有的哺乳动物，包括人类。可以使用疫苗来对抗这种疾病。通过评估体液免疫反应，可以预防这种疾病。最近的报告表明细胞介导的免疫反应（CMI）在评估疫苗效力中的作用。在本研究中，使用介导的新城疫病毒（NDV）菌株R2B产生了两个包含狂犬病病毒糖蛋白G的活载体疫苗候选物，另一个使用具有改变的融合蛋白切割位点的NDV产生了狂犬病病毒糖蛋白G。这些疫苗候选物在实验性小鼠模型中进行测试的功效表明产生了强大的体液和CMI反应。