Association of Apolipoprotein E gene polymorphism with the risk of T2DM and obesity among Egyptian subjects,Gene

当前位置： X-MOL 学术 › Gene › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Association of Apolipoprotein E gene polymorphism with the risk of T2DM and obesity among Egyptian subjects
Gene ( IF 2.6 ) Pub Date : 2020-10-12 , DOI: 10.1016/j.gene.2020.145223
Amr A. Galal , Ahmed A. Abd Elmajeed , Rizk A. Elbaz , Alaa M. Wafa , Rami M. Elshazli

Background

Numerous reports investigated the involvement of apolipoprotein E (APOE) polymorphisms with elevated risk of type 2 diabetes mellitus (T2DM) and obesity. The principal objective of this study is to assess the contribution of APOE polymorphisms (rs429358 and rs7412) with the risk of T2DM and obesity.

Subjects and Methods

This work was designed involving 400 participants [100 healthy controls, 100 T2DM patients, 100 obese patients, and 100 T2DM+obese patients]. Genomic deoxyribonucleic acid (DNA) of the APOE polymorphisms was characterized using the PCR-RFLP assay.

Results

The common predominant genotype of the study population is the APOE Ɛ3/Ɛ3 [T2DM patients (46%), obese patients (52%), T2DM+obese patients (37%), and healthy controls (58%)]. The frequencies of the APOE Ɛ4/Ɛ4 genotype and the APOE*Ɛ4 allele were significantly elevated among T2DM patients (p-value < 0.05). Additionally, the frequencies of the APOE Ɛ2/Ɛ2 genotype and the APOE*Ɛ2 allele were significantly increased among obese patients (p-value < 0.05). Moreover, the frequencies of the APOE Ɛ2/Ɛ2 genotype, APOE*Ɛ2 allele, APOE Ɛ4/Ɛ4 genotype, and APOE*Ɛ4 allele were statistically significant among T2DM+obese patients (p-value < 0.05).

Conclusions

APOE*Ɛ2 and APOE*Ɛ4 alleles were considered as independent risk factor among T2DM+obese patients. Furthermore, the APOE*Ɛ2 allele was correlated with elevated risk of obesity, while the APOE*Ɛ4 allele was correlated with elevated risk of T2DM.

中文翻译：

载脂蛋白E基因多态性与埃及受试者中T2DM和肥胖的风险相关

背景

大量报告调查了载脂蛋白E（APOE）多态性与2型糖尿病（T2DM）和肥胖风险升高的关系。这项研究的主要目的是评估APOE基因多态性（rs429358和rs7412）与2型糖尿病和肥胖症的关系。

主题与方法

这项工作的设计涉及400名参与者[100名健康对照者，100名T2DM患者，100名肥胖患者和100名T2DM +肥胖患者]。使用PCR-RFLP分析对APOE多态性的基因组脱氧核糖核酸（DNA）进行了表征。

结果

该研究人群的常见主要基因型是APOEƐ3/Ɛ3 [T2DM患者（46％），肥胖患者（52％），T2DM +肥胖患者（37％）和健康对照（58％）]。T2DM患者中APOE OE4 /Ɛ4基因型和APOE *Ɛ4等位基因的频率显着升高（p值<0.05）。此外，肥胖患者中APOEƐ2/Ɛ2基因型和APOE *Ɛ2等位基因的频率显着增加（p值<0.05）。此外，APOEƐ2/Ɛ2基因型，APOE *Ɛ2等位基因，APOEƐ4/Ɛ4基因型和APOE *Ɛ4的频率在T2DM +肥胖患者中，等位基因具有统计学意义（p值<0.05）。