Naturally sourced phospholipids are used in many liposomal pharmaceuticals. The present report describes a method to detect the effects of different egg yolk phosphatidylcholines (EPCs) on liposomal physicochemical properties. Five EPC-containing liposomes were prepared using five different EPCs obtained from different suppliers. There was no significant difference in purity between each EPC. The stiffness of the liposomes was examined via atomic force microscopy (AFM) in relation to the liposomal membrane permeability coefficient of encapsulated calcein after gel filtration, which is indicative of liposomal stability including the release of a hydrophilic drug from a liposome. Although the size of the liposome and the encapsulation efficiency of calcein did not significantly change with the type of EPC used, the liposome stiffness was found to vary depending on the EPC used, and liposomes with a similar stiffness were found to show a similar membrane permeability to calcein. Our results indicate the usefulness of stiffness measurement, using AFM as the analytical method, to detect material-derived differences in EPC-containing liposomes that affect drug release from the liposomes. Because drug release is one of the most important liposomal functions, combining this method with other analytical methods could be useful in selecting material for the development and quality control of EPC-containing liposomes.

天然来源的磷脂用于许多脂质体药物。本报告描述了一种检测不同蛋黄磷脂酰胆碱 (EPC) 对脂质体理化特性影响的方法。使用从不同供应商获得的五种不同 EPC 制备了五种含有 EPC 的脂质体。每个 EPC 之间的纯度没有显着差异。脂质体的刚度通过原子力显微镜 (AFM) 与凝胶过滤后封装的钙黄绿素的脂质体膜渗透系数相关，这表明脂质体的稳定性，包括从脂质体中释放亲水性药物。尽管脂质体的大小和钙黄绿素的包封率不会随着所使用的 EPC 类型而显着变化，发现脂质体刚度随所使用的 EPC 而变化，并且发现具有相似刚度的脂质体显示出与钙黄绿素相似的膜渗透性。我们的结果表明刚度测量的有用性，使用 AFM 作为分析方法，检测含 EPC 的脂质体中影响药物从脂质体释放的材料衍生的差异。由于药物释放是最重要的脂质体功能之一，因此将这种方法与其他分析方法相结合可能有助于选择用于开发和质量控制含 EPC 脂质体的材料。检测影响药物从脂质体释放的含有 EPC 的脂质体中材料衍生的差异。由于药物释放是最重要的脂质体功能之一，因此将这种方法与其他分析方法相结合可能有助于选择用于开发和质量控制含 EPC 脂质体的材料。检测影响药物从脂质体释放的含有 EPC 的脂质体中材料衍生的差异。由于药物释放是最重要的脂质体功能之一，因此将这种方法与其他分析方法相结合可能有助于选择用于开发和质量控制含 EPC 脂质体的材料。