Production of hydrogen and acetone-butanol-ethanol (ABE) from hydrolysates of sugarcane bagasse (SCB) and rice-straw (RS) was carried out via dark fermentation using co-culture of Enterobacter aerogenes and Clostridium acetobutylicum. The best improvement in the yield of hydrogen and total ABE production were observed for alkaline pretreated RS at 100 °C for 60 min obtained from the highest total reducing sugars concentration of 40.7 g L⁻¹ after the enzymatic hydrolysis. The hydrolysates of alkaline pretreated samples at 100 °C were efficiently converted to H₂ by co-culture of Enterobacter aerogenes and Clostridium acetobutylicum with the highest hydrogen yield of 136.3 and 149.9 mL H₂ g⁻¹-VS of SCB and RS, representing 3.4 and 1.5-fold enhancement in the hydrogen production yield over that obtained from the pure cultures of Enterobacter aerogenes and Clostridium acetobutylicum, respectively. Mass balance and energy generation analysis indicate that the highest specific ABE and total energy production yields per gram untreated feedstock reached 0.12 g ABE and 5.5 kJ for SCB and 0.08 g ABE and 6.6 kJ for RS.

通过使用产气肠杆菌和丙酮丁醇梭菌的共培养，通过黑暗发酵从甘蔗渣（SCB）和稻草（RS）的水解物中生产氢气和丙酮-丁醇-乙醇（ABE）。在酶水解后，由最高的总还原糖浓度为40.7 g L ^-1获得的碱性预处理的RS在100°C下60分钟观察到氢产率和总ABE产量的最佳改善。通过产气肠杆菌和丙酮丁醇梭菌的共培养，可将碱性预处理样品在100°C时的水解产物有效转化为H ₂。SCB和RS的最高产氢量分别为136.3和149.9 mL H ₂ g ^-1 -VS ，分别比产气肠杆菌和丙酮丁醇梭菌的纯培养物产氢量高3.4和1.5倍。质量平衡和能量生成分析表明，每克未经处理的原料的最高比重ABE和总能量产生量分别为0.12 g ABE和5.5 kJ（对于SCB）和0.08 g ABE和6.6 kJ（对于RS）。