Main conclusion CpGLP1 belongs to the large group of germin-like proteins and comprises a cell wall-localized protein which has superoxide dismutase activity and may contribute towards ROS metabolism and cell wall folding during desiccation. Abstract The plant cell wall is a dynamic matrix and its plasticity is essential for cell growth and processing of environmental signals to cope with stresses. A few so-called resurrection plants like Craterostigma plantagineum survive desiccation by implementing protection mechanisms. In C. plantagineum, the cell wall shrinks and folds upon desiccation to avoid mechanical and oxidative damage which contributes to cell integrity. Despite the high toxic potential, ROS are important molecules for cell wall remodeling processes as they participate in enzymatic reactions and act as signaling molecules. Here we analyzed the C. plantagineum germin-like protein 1 (CpGLP1) to understand its contribution to cell wall folding and desiccation tolerance. The analysis of the CpGLP1 sequence showed that this protein does not fit into the current GLP classification and forms a new group within the Linderniaceae. CpGLP1 transcripts accumulate in leaves in response to dehydration and ABA, and mannitol treatments transiently induce CpGLP1 transcript accumulation supporting the participation of CpGLP1 in desiccation-related processes. CpGLP1 protein from cell wall protein extracts followed transcript accumulation and protein preparations from bacteria overexpressing CpGLP1 showed SOD activity. In agreement with cell wall localization, CpGLP1 interacts with pectins which have not been reported for GLP proteins. Our data support a role for CpGLP1 in the ROS metabolism related to the control of cell wall plasticity during desiccation in C. plantagineum. Electronic supplementary material The online version of this article (10.1007/s00425-020-03485-0) contains supplementary material, which is available to authorized users.

中文翻译：

---

来自 Craterostigma plantagineum 的脱水和 ABA 诱导的胚芽蛋白样蛋白 CpGLP1 具有 SOD 活性，可能有助于干燥过程中细胞壁的完整性


主要结论 CpGLP1 属于一大类胚芽蛋白样蛋白，由细胞壁定位蛋白组成，具有超氧化物歧化酶活性，可能有助于干燥过程中 ROS 代谢和细胞壁折叠。摘要 植物细胞壁是一种动态基质，其可塑性对于细胞生长和处理环境信号以应对胁迫至关重要。一些所谓的复活植物，如 Craterostigma plantagineum，通过实施保护机制在干燥中生存。在车前草中，细胞壁在干燥时收缩和折叠，以避免机械和氧化损伤，这有助于细胞的完整性。尽管具有高毒性，ROS 仍然是细胞壁重塑过程的重要分子，因为它们参与酶促反应并充当信号分子。在这里，我们分析了车前草胚芽蛋白样蛋白 1 (CpGLP1)，以了解其对细胞壁折叠和干燥耐受性的贡献。对 CpGLP1 序列的分析表明，该蛋白不适合当前的 GLP 分类，并在 Linderniaceae 中形成一个新的组。 CpGLP1 转录本在脱水和 ABA 的作用下在叶子中积累，甘露醇处理会短暂诱导 CpGLP1 转录本积累，支持 CpGLP1 参与干燥相关过程。来自细胞壁蛋白提取物的 CpGLP1 蛋白随着转录积累而产生，并且来自过度表达 CpGLP1 的细菌的蛋白制剂显示出 SOD 活性。与细胞壁定位一致，CpGLP1 与果胶相互作用，而 GLP 蛋白尚未有报道。我们的数据支持 CpGLP1 在 ROS 代谢中的作用，该代谢与车前草干燥过程中细胞壁可塑性的控制有关。 电子补充材料 本文的在线版本 (10.1007/s00425-020-03485-0) 包含补充材料，可供授权用户使用。