This study aimed to investigate the effect of LyPB on the intestinal microflora of giant pandas with indigestion, using high-throughput sequencing (HTS) technology. The species distribution and microfloral density and diversity before and after administration of the LyPB probiotic agent were analyzed. LyPB evidently has the ability to adjust the floral imbalance in the panda’s intestine. To test the effects of LyPB on the microflora of the panda gut, fecal samples were taken from a healthy giant panda (Anan) without administration of LyPB and from a dyspeptic giant panda Yangyang before and after LyPB administration. Compared with the sample obtained from healthy Anan (anan-c) and that obtained from dyspeptic Yangyang before LyPB administration (yangyang1), the sample taken from Yangyang (yangyang2) after LyPB administration displayed a significant increase in the operational taxonomic unit index. An increase in the Chao index indicated an increase in the microfloral richness, while an increase in the Shannon index indicated an increase in microfloral diversity. At phylum and genus levels, a significant increase was observed in the density of probiotic bacteria of phylum firmicutes, genus Streptococcus, while a drastic reduction in the density of Escherichia coli/Escherichia coli Shigella/bacteria of genus Shigella was observed. Data obtained in this study shows that LyPB preparations successfully improve the microbial structure within the panda’s intestinal canal by significantly increasing the effective microbial community and decreasing the number of pathogenic microbes.

本研究旨在通过高通量测序（HTS）技术研究LyPB对消化不良大熊猫肠道菌群的影响。分析了LyPB益生菌剂给药前后的物种分布，菌群密度和多样性。LyPB显然具有调节熊猫肠道中花卉不平衡的能力。为了测试LyPB对大肠肠道菌群的影响，在不施用LyPB的情况下从健康的大熊猫（Anan）和在LyPB施用前后，从消化不良的大熊猫阳阳采集粪便样品。与从健康的Anan（anan-c）和消化不良的Yangyang（在LyPB给药前）采集的样品相比（yangyang1），LyPB管理后从阳阳（yangyang2）采集的样本显示可操作分类单位指数显着增加。朝指数的增加表明微生物丰富度的增加，而香农指数的增加表明微生物多样性的增加。在门和属水平上，观察到硬门门属的益生菌的密度显着增加。链球菌，而在密度的显着降低的大肠杆菌/大肠杆菌志贺氏菌/属的细菌痢疾杆菌中观察到。这项研究获得的数据表明，LyPB制剂通过显着增加有效微生物群落并减少病原微生物数量，成功改善了熊猫肠道内的微生物结构。