Whitefly is an important pest and vector of many plant begomoviruses. Investigations were carried out to understand the acquisition, retention, transmission, and gender basis transmission efficiency of Mesta yellow vein mosaic virus (MeYVMV) by whitefly. The minimum acquisition access feeding period (AAFP) was 0.10 h which resulted in 11.1% infected plants. Based on LAMP assay and yellowing symptoms, 100% virus acquisition was achieved by whitefly with an exposure of 18 to 48 h. Virus was transmitted in tested plants up to 88.8% after 1 to 10 days retention time of viruliferous whiteflies, whereas no symptoms were observed after 35 days. Fifteen minutes of exposure to viruliferous whiteflies on healthy plants resulted in 11.1% transmission. Cent percent transmission of virus was achieved in plants with 12 h exposure period. Highest virus transmission efficiency of 55.60% was observed in female whitefly compared to 33.3% in single male whitefly. Present study shall help to understand the interaction of insect-plant–virus relationship, epidemiological knowledge, and formulation of management strategies against virus and its vector.

中文翻译：

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Mesta 黄脉花叶病毒：应用环介导等温扩增方法研究烟粉虱（半翅目：粉虱科）在红麻中的获取、保留和传播效率

粉虱是许多植物 begomoviruses 的重要害虫和载体。开展调查以了解粉虱对 Mesta 黄脉花叶病毒 (MeYVMV) 的获取、保留、传播和性别传播效率。最小获取访问饲养期 (AAFP) 为 0.10 小时，导致 11.1% 的植物受感染。根据 LAMP 测定和变黄症状，粉虱在 18 至 48 小时的暴露下实现了 100% 的病毒获取。在带毒粉虱保留 1 至 10 天后，病毒在受试植物中的传播率高达 88.8%，而在 35 天后未观察到任何症状。在健康植物上接触带毒粉虱 15 分钟导致 11.1% 的传播。在暴露时间为 12 小时的植物中实现了百分之百的病毒传播。在雌性粉虱中观察到的最高病毒传播效率为 55.60%，而在单只雄性粉虱中为 33.3%。本研究将有助于了解昆虫-植物-病毒关系、流行病学知识以及制定针对病毒及其载体的管理策略的相互作用。