Rapid spread of vector borne diseases, especially through mosquitoes is in alarming rise worldwide. Among mosquitoes, Aedes aegypti is a major vector transmitting arboviruses such as dengue and zika to human population. The ineffectiveness of control measures against these arboviruses has necessitated alternate strategies to curb disease transmission. Recent studies show that symbiotic bacteria from insects hinder pathogen transmission by altering vector competence. Therefore, analyzing the bacterial communities of mosquitoes is essential to identify potential bacteria that play a critical role in vector competence. Salivary gland, a key organ in viral transmission however, is minimally explored towards their microbiota. In this study, we have screened 28 bacterial isolates from salivary glands of non-blood fed (NBF) and blood fed (BF) female Ae. aegypti, collected from the field population of Coimbatore, India. Among them, 11 bacterial species from distinct genera belonging to three major phyla; Proteobacteria, Firmicutes and Actinobacteria were identified through 16S rRNA gene sequencing. Interestingly, the differences in bacterial species associated with salivary glands were clearly discernible. This suggests that the dynamics of bacterial population after blood feeding is not restricted to the mosquito gut, but envisioned in the salivary glands as well. Overall, the results shed more light into the understating on salivary glands microbiota of dreadful vector Ae. aegypti.

中文翻译：

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与埃及伊蚊唾液腺相关的可培养细菌群落

病媒传播疾病的迅速传播，尤其是通过蚊子传播的疾病在世界范围内呈惊人的上升趋势。在蚊子中，埃及伊蚊是将登革热和寨卡病毒等虫媒病毒传播给人类的主要媒介。针对这些虫媒病毒的控制措施无效，因此需要采取替代策略来遏制疾病传播。最近的研究表明，来自昆虫的共生细菌通过改变载体能力来阻碍病原体的传播。因此，分析蚊子的细菌群落对于识别在载体能力中起关键作用的潜在细菌至关重要。然而，唾液腺是病毒传播的关键器官，对其微生物群的探索很少。在这项研究中，我们从非血饲 (NBF) 和血饲 (BF) 雌性伊蚊的唾液腺中筛选了 28 株细菌。aegypti，收集自印度哥印拜陀的野外种群。其中，11个细菌属不同属，分属三大门；Proteobacteria、Firmicutes 和 Actinobacteria 通过 16S rRNA 基因测序鉴定。有趣的是，与唾液腺相关的细菌种类的差异是清晰可辨的。这表明吸血后细菌种群的动态不仅限于蚊子的肠道，而且也存在于唾液腺中。总体而言，这些结果进一步揭示了对可怕载体 Ae 唾液腺微生物群的低估。埃及人。通过 16S rRNA 基因测序鉴定厚壁菌门和放线菌门。有趣的是，与唾液腺相关的细菌种类的差异是清晰可辨的。这表明吸血后细菌种群的动态不仅限于蚊子的肠道，而且也存在于唾液腺中。总体而言，这些结果进一步揭示了对可怕载体 Ae 唾液腺微生物群的低估。埃及人。通过 16S rRNA 基因测序鉴定厚壁菌门和放线菌门。有趣的是，与唾液腺相关的细菌种类的差异是清晰可辨的。这表明吸血后细菌种群的动态不仅限于蚊子的肠道，而且也存在于唾液腺中。总体而言，这些结果进一步揭示了对可怕载体 Ae 唾液腺微生物群的低估。埃及人。结果进一步揭示了对可怕载体 Ae 唾液腺微生物群的低估。埃及人。结果进一步揭示了对可怕载体 Ae 唾液腺微生物群的低估。埃及人。