The vector-borne bacterium Xylella fastidiosa subsp. pauca is the etiological agent of important plant diseases, such as citrus variegated chlorosis (CVC). Biological studies are relatively scarce for CVC, in part because citrus is a low titer woody host, in which X. fastidiosa has a long incubation period and low transmission efficiency by vectors. To optimize transmission bioassays, this study was conducted to: i) identify herbaceous plants that allow rapid multiplication and systemic colonization of CVC strains; and ii) evaluate the herbaceous hosts for feeding and survival of the sharpshooter vector, Bucephalogonia xanthophis (Berg), and as indicators of infection after vector inoculation. Mechanical inoculations showed systemic colonization of X. fastidiosa in seven plant species, with higher rates of infection mainly in Catharanthus roseus. The lowest vector mortality was observed on C. roseus, Medicago sativa and Ocimum basilicum. The highest feeding rate was verified on O. basilicum, followed by M. sativa and C. roseus. Infections after vector inoculation were detected in C. roseus, M. sativa, O. basilicum and S. americanum. Among the plant species tested, C. roseus proved to be the most suitable experimental plant for transmission assays with CVC strains of X. fastidiosa subsp. pauca.

载体传播的细菌Xylella fastidiosa亚种。pauca是重要植物病害的病原体，例如柑桔杂色萎黄病（CVC）。CVC的生物学研究相对较少，部分原因是柑橘是低滴度木本宿主，其中X. fastidiosa的潜伏期长，通过载体的传播效率低。为了优化传播生物测定，进行了这项研究以：i）鉴定能够快速繁殖和系统定殖CVC菌株的草本植物；ii）评估草本宿主对神枪手载体黄头虫的摄食和存活的影响（Berg），并作为载体接种后的感染指标。机械接种表现出系统性定植X. fastidiosa七种植物物种，主要分布在感染率较高长春花。在玫瑰色念珠菌，紫花苜蓿和罗勒叶上观察到最低的病媒死亡率。最高的进食率在O. basilicum上得到证实，其次是M. sativa和C. roseus。接种载体后，在玫瑰色念珠菌，苜蓿分支杆菌，罗勒草和美洲链球菌中检测到感染。。在所测试的植物物种中，玫瑰色念珠菌被证明是最合适的实验植物，用于使用法氏假单胞菌亚种的CVC菌株进行传播测定。包卡。