Abstract—In recent years, the demand for technical ethanol has increased due to its use in the transport sector. Xylose is the major five-carbon sugar obtained as a result of lignocellulose hydrolysis; however, the industrial producer of alcohol S. cerevisiae yeast ferments exclusively hexoses. Based on a recombinant strain capable of xylose metabolism, the derivatives with increased expression of the ZNF1 gene and deletion of the SIP4 gene encoding transcription factors were constructed. It was found that overexpression of the ZNF1 gene did not affect the fermentation of glucose or xylose. The deletion of the SIP4 gene did not affect the fermentation of glucose but resulted in a 29% decrease in ethanol production during xylose fermentation in comparison with the parental strain.

摘要—近年来，由于在运输领域的使用，对工业用乙醇的需求增加了。木糖是木质纤维素水解的主要五碳糖。但是，酿酒酵母的工业生产商只能发酵己糖。基于具有木糖代谢能力的重组菌株，构建了ZNF1基因表达增强和SIP4基因编码转录因子缺失的衍生物。发现ZNF1基因的过表达不影响葡萄糖或木糖的发酵。SIP4的删除 该基因不影响葡萄糖的发酵，但是与亲本菌株相比，导致木糖发酵过程中乙醇产量降低29％。