The aim of this study to investigate the potential effects of essential oils and compounds obtained from MC fruit on sepsis induced endothelial cell damage in human umbilical cord vein endothelial cells (HUVECs) at molecular and cellular levels on in vitro sepsis model. A sepsis model was induced by the application of LPS. The HUVEC treatment groups were as follows: control, LPS, MC, MC plus LPS, 1.8 cineole, 1.8 cineole plus LPS, α-pinene, α-pinene plus LPS, α-terpineol, and α-terpineol plus LPS. Following the treatments, cell proliferation was analyzed using the xCELLigence® system. The mRNA expression of various cytokines [tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), and IL-6] and endothelial nitric oxide (eNOS) were determined by quantitative polymerase chain reaction (qPCR) analysis. The 1.8 cineole and α-pinene treatments at specific doses showed toxic effects on α-terpineine, although it did not result in a change in the cellular index as compared with that of the control group. The application of LPS to HUVECs led to a significant decrease in the cellular index, depending on the treatment time. It did not correct the decreased cell index of MC plus LPS and α-terpineol plus LPS groups as compared with that of the LPS-only group. The 1.8 cineole plus LPS treatment and α-pinene plus LPS treatment significantly increased the cell index as compared with that of the LPS-only treatment, and the cell index in these groups was closer to that of the control. According to the results of the qPCR analysis, neither the MC-only treatment nor the α-terpineol-only treatment significantly reduced cellular damage caused by LPS-induced increases in TNF-α, IL-1β, IL-6, and eNOS mRNA expression. However, both the 1.8 cineole treatment and α-pinene treatments significantly decreased TNF-α, IL-1β, IL-6, and eNOS mRNA expression induced by LPS. Volatile oil obtained from MC fruit and the MC compound α-terpineol had no effect on the decreased cell index and increased cytokine response due to LPS-induced endothelial cell damage. However, 1.8 cineole and α-pinene, other major components of MC fruit, ameliorated LPS-induced damage in HUVECs at cellular and biomolecular levels (TNF-α, IL-1β, IL-6, and eNOS).

这项研究的目的是在体外败血症模型上研究从MC果实中提取的精油和化合物对脓毒症诱导的人脐静脉血管内皮细胞（HUVEC）内皮细胞损伤的潜在影响。通过应用LPS诱发败血症模型。HUVEC治疗组如下：对照组，LPS，MC，MC加LPS，1.8 cineole，1.8 cineole加LPS，α-pine烯，α-pine烯加LPS，α-松油醇和α-松油醇加LPS。处理后，使用xCELLigence®系统分析细胞增殖。通过定量聚合酶链反应（qPCR）分析确定各种细胞因子[肿瘤坏死因子（TNF-α），白介素-1β（IL-1β）和IL-6]和内皮型一氧化氮（eNOS）的mRNA表达。1。尽管没有导致与对照组相比的细胞指数变化，但是8种特定剂量的桉树脑和α-pine烯处理显示出对α-萜品酸的毒性作用。将LPS应用于HUVEC会导致细胞指数显着降低，具体取决于治疗时间。与仅使用LPS的组相比，它不能纠正MC加LPS和α-松油醇加LPS组降低的细胞指数。与仅使用LPS的处理相比，1.8 cineole加LPS处理和α-pinene加LPS处理显着增加了细胞指数，并且这些组中的细胞指数均接近于对照组。根据qPCR分析的结果，仅MC治疗和仅α-松油醇治疗均不能显着降低由LPS诱导的TNF-α，IL-1β，IL-6和eNOS mRNA表达增加引起的细胞损伤。但是，1.8桉树脑处理和α-pine烯处理均显着降低LPS诱导的TNF-α，IL-1β，IL-6和eNOS mRNA表达。从MC果实和MC化合物α-松油醇中获得的挥发油对LPS诱导的内皮细胞损伤所致的细胞指数降低和细胞因子应答增加没有影响。然而，MC果实的其他主要成分1.8桉树脑和α-pine烯在细胞和生物分子水平（TNF-α，IL-1β，IL-6和eNOS）上减轻了LPS诱导的HUVEC损伤。和LPS诱导的eNOS mRNA表达。从MC果实和MC化合物α-松油醇中获得的挥发油对LPS诱导的内皮细胞损伤所致的细胞指数降低和细胞因子应答增加没有影响。然而，MC果实的其他主要成分1.8桉树脑和α-pine烯在细胞和生物分子水平（TNF-α，IL-1β，IL-6和eNOS）上减轻了LPS诱导的HUVEC损伤。和LPS诱导的eNOS mRNA表达。从MC果实和MC化合物α-松油醇中获得的挥发油对LPS诱导的内皮细胞损伤所致的细胞指数降低和细胞因子应答增加没有影响。然而，MC果实的其他主要成分1.8桉树脑和α-pine烯在细胞和生物分子水平（TNF-α，IL-1β，IL-6和eNOS）上减轻了LPS诱导的HUVEC损伤。