Abstract

Due to the high mortality rate and an increase in breast cancer incidence, it has been challenging for researchers to come across an effective chemotherapeutic strategy with minimum side effects. Therefore, the need for the development of effective chemotherapeutic drugs is still on the verge. Consequently, we approached a new mechanism to address this issue. The naturally available peptide named latcripin-7A (LP-7A), extracted from a mushroom called Lentinula edodes, provided us promising results in terms of growth arrest, apoptosis, and autophagy in breast cancer cells (MCF-7 and MDA-MB-231). Expressions of protein markers for apoptosis, autophagy, and cell cycle were confirmed via Western blot analysis. Migration and invasion assays were performed to analyze the anti-migratory and anti-invasive properties of LP-7A, while cell cycle analysis was performed via flow cytometry to evaluate its affect over cell growth. Supportive assays were performed like acridine orange, Hoechst 33258 stain, DNA fragmentation, and mitochondrial membrane potential (MMP) to further confirm the anticancer effect of LP-7A on breast cancer cell lines. It is concluded that LP-7A effectively reduces migration and promotes apoptosis as well as autophagy in MCF-7 and MDA-MB-231 breast cancer cell lines by inducing cell growth arrest at G₀/G₁ phase and decreasing mitochondrial membrane potential without adverse effects on MCF-10A normal breast cells.

Key points

• In this study, we have investigated the anti-cancer activity of novel latcripin-7A (LP-7A), a protein extracted as a result of de novo characterization of Lentinula edodes C_91–3.

• We conclude in our research work that LP-7A can initiate diverse cell death-related events, i.e., apoptosis and autophagy in both triple-positive and triple-negative breast cancer cell lines by interacting with different nodes of cellular signaling that can further be investigated in vivo to gain a better understanding.

中文翻译：

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Latcripin-7A，香菇C 91–3的衍生物，减少迁移并诱导乳腺癌细胞G 1期的凋亡，自噬和细胞周期停滞

摘要

由于高死亡率和乳腺癌发病率的增加，研究人员要想出一种具有最小副作用的有效化疗策略一直是一项挑战。因此，仍然迫切需要开发有效的化学治疗药物。因此，我们采用了一种新的机制来解决此问题。从名为香菇香菇的蘑菇中提取的天然肽latcripin-7A（LP-7A）在乳腺癌细胞（MCF-7和MDA-MB-231）的生长停滞，凋亡和自噬方面，我们提供了令人鼓舞的结果。通过蛋白质印迹分析证实了用于凋亡，自噬和细胞周期的蛋白质标志物的表达。进行迁移和侵袭分析以分析LP-7A的抗迁移和抗侵袭特性，同时通过流式细胞仪进行细胞周期分析以评估其对细胞生长的影响。进行了支持性测定，如a啶橙，Hoechst 33258染色，DNA片段化和线粒体膜电位（MMP），以进一步证实LP-7A对乳腺癌细胞系的抗癌作用。₀ / G ₁期，线粒体膜电位降低，而对MCF-10A正常乳腺细胞无不良影响。

关键点

•在这项研究中，我们研究了新型latcripin-7A（LP-7A）的抗癌活性，该蛋白是从香菇C _91-3的从头表征中_提取的。

•我们在研究工作中得出结论，LP-7A可以通过与细胞信号转导的不同节点相互作用而引发多种细胞死亡相关事件，即三阳性和三阴性乳腺癌细胞系的凋亡和自噬。进行体内调查以获得更好的了解。