We studied the allopatric complex Pulsatilla patens (L.) Mill. s.lat. (Ranunculaceae) in north-eastern European Russia and the Urals. In this region, there are two kinds of P. patens with different perianth colours in monochrome and polychrome populations. To clarify their taxonomic boundaries, we used the sequences of chloroplast DNA (rbcL and matK) and nuclear DNA (ITS2), in addition to morphological characteristics. The combination of three markers (rbcL+matK+ITS2) was found to be the most effective for phylogenetic resolution. The samples of two morphologically-different taxa P. uralensis and P. patents s.str. were shown to form a single clade on the phylogenetic tree. Based on the molecular phylogenetic analysis, we were not able to unequivocally prove the independent existence of P. uralensis.

中文翻译：

---

白头翁和P. patens s.str。的系统发生关系和分类单元的状态。（毛un科）在欧洲东北部的俄罗斯

我们研究了异特异复合物白头翁（L.）Mill。拉特 （毛un科）在欧洲东北部的俄罗斯和乌拉尔。在该区域中，单色和多色种群中有两种花被色不同的紫菜。为了阐明它们的分类学边界，我们使用了叶绿体DNA（rbcL和matK）和核DNA（ITS2）的序列，以及形态特征。发现三种标记物（rbcL + matK + ITS2）的组合对于系统发育分辨最有效。两种形态不同的类群P. uralensis和P. Patent s.str。的样本。被证明在系统发育树上形成单个进化枝。基于分子系统发育分析，我们不能明确地证明P. uralensis的独立存在。