Restoring bone defects are the major challenge facing clinical trial therapy, particularly skull related problems. Morin, a naturally occurring compound, has pro-osteogenesis. This research focuses on assessing the role of morin for its pro-osteogenesis activities. We utilized in vivo and in vitro models to investigate the molecular-level mechanisms of morin’s osteoblastic biological activity. The effectiveness of morin on pro-osteogenesis (100 mg/kg/day) was assessed by monitoring modifications in the bone histomorphometry score, the development of immature osteoblasts from mesenchymal stems cells and improvements in the expression of pro-osteogenic cytokines in skull defected (SD) mice. Quantitative—PCR, Western blot analysis, and immunofluorescence were studied to investigate the signaling pathways. Morin has a substantial in vivo pro-osteogenesis effect which can facilitate the development of osteoblasts, the production of osteoblast related marker genes, and in vitro protein markers for osteoblasts. From a molecular biology standpoint, morin contributes to the development of osteoblasts and stimulation of the Wnt pathway with the activation and translocation of β-catenin nuclei. Our findings from the study revealed that morin may be a beneficial substitute for helping regenerate bone defects.

恢复骨缺损是临床试验治疗面临的主要挑战，特别是与颅骨相关的问题。桑色素是一种天然存在的化合物，具有促骨生成作用。本研究的重点是评估桑色素在促骨生成活性中的作用。我们利用体内和体外模型研究桑色素成骨细胞生物活性的分子水平机制。通过监测骨组织形态计量学评分的变化、间充质干细胞未成熟成骨细胞的发育以及缺损颅骨中促成骨细胞因子表达的改善，评估桑色素对促骨生成的有效性（100 mg/kg/天）。 SD）小鼠。定量——PCR、蛋白质印迹分析和免疫荧光研究了信号传导途径。桑色素具有显着的体内促骨作用，可以促进成骨细胞的发育、成骨细胞相关标记基因的产生以及成骨细胞的体外蛋白质标记。从分子生物学的角度来看，桑色素有助于成骨细胞的发育，并通过 β-连环蛋白核的激活和易位刺激 Wnt 通路。我们的研究结果表明，桑色素可能是帮助骨缺损再生的有益替代品。