Single-molecule super-resolution microscopy has developed from a specialized technique into one of the most versatile and powerful imaging methods of the nanoscale over the past two decades. In this perspective, we provide a brief overview of the historical development of the field, the fundamental concepts, the methodology required to obtain maximum quantitative information, and the current state of the art. Then, we will discuss emerging perspectives and areas where innovation and further improvement are needed. Despite the tremendous progress, the full potential of single-molecule super-resolution microscopy is yet to be realized, which will be enabled by the research ahead of us.

中文翻译：

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生物学及其他领域的单分子超分辨率显微镜——基本要素、当前趋势和未来挑战

在过去的二十年里，单分子超分辨率显微镜已经从一种专业技术发展成为纳米级最通用和最强大的成像方法之一。从这个角度来看，我们简要概述了该领域的历史发展、基本概念、获得最大定量信息所需的方法以及当前的技术水平。然后，我们将讨论需要创新和进一步改进的新兴观点和领域。尽管取得了巨大进展，但单分子超分辨率显微镜的全部潜力尚未实现，这将通过我们面前的研究实现。