Chronic osteomyelitis in presence of orthopedic implants is a condition observed in the field of biomaterials as it impairs early bone-implant contact, fixation and integration. In this study, a surgical intramedullary tibial insertion was performed using a titanium wire previously inoculated with Staphylococcus aureus in order to develop an osteomyelitis model in a clinically relevant long bone and in absence of any prophylactic treatment. As such, twenty-two male Sprague-Dawley rats received a sterile or inoculated intramedullary biomaterial with either 2 × 106 or 1 × 107 S. aureus colony forming units. Bacterial burden, inflammation, morphological changes, as well as newly formed bone tissues were evaluated for histopathology following a period of either eight or fifteen days of implantation. The implant inoculated in presence of the highest bacterial load was effective to produce significant periprosthetic infection observations in addition to hard and soft tissue inflammation consistent with the development of osteomyelitis. In contrast, neither the sterile nor the low-dose implant inoculation showed inflammation and clinical infection signs, but rather produced an expected bone remodeling and appropriate healing associated with biomaterial implantation. Complete health assessment is presented with histopathological periprosthetic results.

中文翻译：

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使用胫骨髓内植入物接种急性啮齿动物骨髓炎感染模型的表征

存在骨科植入物的慢性骨髓炎是生物材料领域中观察到的一种疾病，因为它会损害早期骨与植入物的接触、固定和整合。在这项研究中，为了在没有任何预防性治疗的情况下在临床相关的长骨中建立骨髓炎模型，使用先前接种过金黄色葡萄球菌的钛丝进行胫骨髓内手术。因此，22 只雄性 Sprague-Dawley 大鼠接受了无菌或接种的髓内生物材料，其中含有 2 × 106 或 1 × 107 金黄色葡萄球菌菌落形成单位。在植入 8 天或 15 天后，对细菌负荷、炎症、形态变化以及新形成的骨组织进行组织病理学评估。除了与骨髓炎的发展一致的硬组织和软组织炎症外，在存在最高细菌负荷的情况下接种的植入物还可以有效地产生显着的假体周围感染观察结果。相比之下，无菌和低剂量植入物接种均未显示炎症和临床感染迹象，而是产生了与生物材料植入相关的预期骨重塑和适当愈合。完整的健康评估与组织病理学假体周围结果一起呈现。无菌或低剂量植入物接种均未显示炎症和临床感染迹象，而是产生了与生物材料植入相关的预期骨重塑和适当愈合。完整的健康评估与组织病理学假体周围结果一起呈现。无菌或低剂量植入物接种均未显示炎症和临床感染迹象，而是产生了与生物材料植入相关的预期骨重塑和适当愈合。完整的健康评估与组织病理学假体周围结果一起呈现。