Pseudomonas aeruginosa metabolizes pyocyanin, a redox molecule related to diverse biological activities. Culture conditions for the production of pyocyanin in a defined medium were optimized using a statistical design and response surface methodology. The obtained conditions were replicated using as substrate an alkaline residual liquid of cooked maize and its by-products. The untreated effluent (raw nejayote, RN) was processed to obtain a fraction without insoluble solids (clarified fraction, CL), then separated by a 30 kDa membrane where two fractions, namely, retentate (RE) and filtered (FI), were obtained. Optimal conditions in the defined medium were 29.6 °C, 223.7 rpm and pH = 6.92, which produced 2.21 μg mL⁻¹ of pyocyanin, and by using the wastewater, it was possible to obtain 3.25 μg mL⁻¹ of pyocyanin in the retentate fraction at 40 h. The retentate fraction presented the highest concentration of total solids related to the maximum concentration of pyocyanin (PYO) obtained. The pyocyanin redox behavior was analyzed using electrochemical techniques. In this way, valorization of lime-cooked maize wastewater (nejayote) used as a substrate was demonstrated in the production of a value-added compound, such as pyocyanin, a redox metabolite of Pseudomonas aeruginosa NEJ01R.

中文翻译：

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利用玉米副产物优化铜绿假单胞菌NEJ01R生产氧化还原代谢产物（花青素）的方法

铜绿假单胞菌代谢花青素，一种与多种生物活性有关的氧化还原分子。使用统计设计和响应面方法优化了在定义的培养基中生产黄绿素的培养条件。使用煮熟的玉米及其副产物的碱性残留液体作为底物，复制获得的条件。处理未经处理的流出物（未加工的Nejayote，RN）以获得不含不溶性固体的馏分（澄清馏分，CL），然后用30 kDa膜分离，得到两个馏分，即截留物（RE）和过滤（FI） 。在定义的培养基中的最佳条件是29.6°C，223.7 rpm和pH = 6.92，可产生2.21μgmL ^-1的花青素，通过使用废水，有可能获得3.25μgmL在40 h时，渗余物级分中的pyocyanin ^-1。截留物部分的总固体含量最高，与所获得的绿脓素（PYO）的最大浓度有关。使用电化学技术分析了花青素的氧化还原行为。以此方式，在增值化合物例如绿脓杆菌绿脓杆菌NEJ01R的氧化还原代谢产物-氰化色素的生产中，证明了用作基质的石灰熟玉米废水（nejayote）的增值作用。