To explore the characteristics of mitogenomes and reveal phylogenetic relationships of the tribes of Zyginellini and Typhlocybini in Typhlocybinae, mitogenomes of two species of the Zyginellini, Parazyginella tiani and Limassolla sp., were sequenced. Mitogenomes of both species contain 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a large non-coding region (A + T-rich region). These characteristics are similar to other Membracoidea mitogenomes. All PCGs initiate with the standard start codon of ATN and terminate with the complete stop codon of TAA/G or with an incomplete T codon. All tRNAs have the typical clover-leaf structure, except trnS1 which has a reduced DHU arm and the acceptor stem of trnR is 5 or 6 bp in some species, an unusual feature here reported for the first time in Typhlocybinae. The A + T-rich region is highly variable in length and in numbers of tandem repeats present. Our analyses indicate that nad6 and atp6 exhibit higher evolutionary rates compared to other PCGs. Phylogenetic analyses by both maximum likelihood and Bayesian methods based on 13 protein-coding genes of 12 species of Typhlocybinae suggest that Zyginellini are paraphyletic with respect to Typhlocybini.

中文翻译：

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两个物种完整线粒体基因组的表征及Zyginellini（半翅目：叶蝉科：叶蝉亚科）系统发育状况的初步调查


探讨斑蝽亚科斑蝽亚科斑蝽属和斑蝽属的线粒体基因组特征，揭示斑蝽亚科斑斑蝽属两个种的线粒体基因组的系统发育关系。 蒂亚尼 和 利马索拉 sp.，进行了测序。两个物种的线粒体基因组均含有 13 个蛋白质编码基因 (PCG)、22 个转移 RNA 基因 (tRNA)、两个核糖体 RNA 基因 (rRNA) 和一个大的非编码区（A + T 丰富区）。这些特征与其他膜科有丝分裂基因组相似。所有PCG均以ATN的标准起始密码子起始，并以TAA/G的完整终止密码子或不完整的T密码子终止。所有 tRNA 均具有典型的三叶草结构，但trnS1除外，其 DHU 臂缩短，并且trnR的受体茎在某些物种中为 5 或 6 bp，这是首次在 Typhlocybinae 中报道的不寻常特征。富含 A + T 的区域的长度和串联重复的数量变化很大。我们的分析表明，与其他 PCG 相比， nad6和atp6表现出更高的进化速率。基于 12 个 Typhlocybini 物种的 13 个蛋白质编码基因，采用最大似然法和贝叶斯方法进行的系统发育分析表明，Zyginellini 与 Typhlocybini 是并系群。