To explore the characteristics of mitogenomes and reveal phylogenetic relationships of the tribes of Zyginellini and Typhlocybini in Typhlocybinae, mitogenomes of two species of the Zyginellini, Parazyginella tiani and Limassolla sp., were sequenced. Mitogenomes of both species contain 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a large non-coding region (A + T-rich region). These characteristics are similar to other Membracoidea mitogenomes. All PCGs initiate with the standard start codon of ATN and terminate with the complete stop codon of TAA/G or with an incomplete T codon. All tRNAs have the typical clover-leaf structure, except trnS1 which has a reduced DHU arm and the acceptor stem of trnR is 5 or 6 bp in some species, an unusual feature here reported for the first time in Typhlocybinae. The A + T-rich region is highly variable in length and in numbers of tandem repeats present. Our analyses indicate that nad6 and atp6 exhibit higher evolutionary rates compared to other PCGs. Phylogenetic analyses by both maximum likelihood and Bayesian methods based on 13 protein-coding genes of 12 species of Typhlocybinae suggest that Zyginellini are paraphyletic with respect to Typhlocybini.

中文翻译：

---

两个物种的完整线粒体基因组的特征与Zyginellini（半翅目：cade科：typhlocybinae）的系统发生状况的初步调查。

为了探究有丝分裂基因组的特征并揭示斑潜蝇属中的Zyginellini和Typhlocybini部落的系统发育关系，对Zyginellini的两种物种tiantian和Limassolla sp。的有丝分裂基因组进行了测序。这两个物种的基因组均包含13个蛋白质编码基因（PCG），22个转移RNA基因（tRNA），两个核糖体RNA基因（rRNA）和一个较大的非编码区（富含A + T的区域）。这些特征与其他Membracoidea有丝分裂基因组相似。所有PCG均以ATN的标准起始密码子开始，并以TAA / G的完全终止密码子或不完整的T密码子终止。除trnS1具有减少的DHU臂和受体的茎外，所有tRNA均具有典型的三叶草叶结构。 在某些物种中，trnR为5或6 bp，这是一种在Typholocybinae中首次报道的异常特征。富含A + T的区域在长度和串联重复数方面变化很大。我们的分析表明，nad6和atp6与其他PCG相比具有更高的进化速率。基于最大可能性和贝叶斯方法的系统发育分析，基于12种typhlocybinae的13个蛋白质编码基因，表明Zyginellini与Typhlocybini有共生关系。