The present study aimed to investigate the expression levels and clinical significance of the calcineurin B homologous protein 2 (CHP2) in non–small cell lung cancer (NSCLC), and to study its effects on biological characteristics of NSCLC cells. Tumor and adjacent samples were collected from 196 NSCLC patients. Western blot analysis was used to detect the expression levels of the CHP2 in 8 pairs of NSCLC fresh tissues and 4 NSCLC cell lines. Immunohistochemical analysis was used to detect the expression of the CHP2 in 188 additional pairs of NSCLC wax block tissues. The data indicated that the expression levels of the CHP2 in the paraffin and fresh tissues of NSCLC were significantly higher than those of the adjacent tissues. According to the histo-score, univariate and multivariate analysis indicated that a high expression level of CHP2 was an important factor affecting the 5-year survival rate of NSCLC patients. After knocking down the expression of CHP2 in NSCLC cell lines, the proliferative, migratory, and invasive activities of NSCLC-CHP2 cells were decreased which were assessed by Western blotting, Cell Counting Kit-8, and transwell and wound-healing assays. In conclusion, the data demonstrated that CHP2 was highly expressed in NSCLC and that it could promote the development of NSCLC, suggesting its potential application for the therapy of NSCLC.

中文翻译：

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CHP2 参与非小细胞肺癌的发生和患者的不良预后


本研究旨在探讨钙调神经磷酸酶B同源蛋白2（CHP2）在非小细胞肺癌（NSCLC）中的表达水平和临床意义，并研究其对NSCLC细胞生物学特性的影响。肿瘤和癌旁样本采集自 196 名 NSCLC 患者。 Western blot分析检测8对NSCLC新鲜组织和4个NSCLC细胞系中CHP2的表达水平。采用免疫组织化学分析检测另外 188 对 NSCLC 蜡块组织中 CHP2 的表达。数据表明，NSCLC石蜡组织和新鲜组织中CHP2的表达水平显着高于癌旁组织。根据组织评分，单因素和多因素分析表明CHP2的高表达水平是影响NSCLC患者5年生存率的重要因素。敲低 NSCLC 细胞系中 CHP2 的表达后，通过 Western blotting、Cell Counting Kit-8、transwell 和伤口愈合实验评估 NSCLC-CHP2 细胞的增殖、迁移和侵袭活性降低。总之，数据表明CHP2在NSCLC中高表达，并且可以促进NSCLC的发展，提示其在NSCLC治疗中的潜在应用。