Abstract

Over the last few years, nanofibrous mats produced by electrospinning technique showed potential feasibility for skin repairing. It is expected that incorporation of plant extracts into such structures could provide a better performance for biomedical and wound healing applications. The present study deals with the fabrication of PVA based nanofiber mats loaded with the extract of a traditional plant called Nigella sativa to enhance the antibacterial, moisture management and wound healing properties. The structure, bonding behavior and moisture management properties of the developed electrospun nanofibers have been investigated. Besides, the antibacterial activity against Staphylococcus aureus (S. aureus) and Escrcherichia coli (E. coli) bacteria and wound healing activity of the abovementioned mats were also studied. The incorporation of Nigella sativa extract into PVA mats exhibited an excellent antibacterial activity with the formation of inhibition zone of 10.27 mm and 7.8 mm against both bacterial cells. It was observed that the developed mats quickly healed the wound by forming network structure on the skin. The SEM analysis showed the formation of nanofiber having diameter of 178.8 ± 16.9 nm and the presence of Nigella sativa extract in the electrospun fibers was confirmed by FTIR. Besides, the developed mat was classified as water penetrated according to AATCC test characteristics. Thus, the newly developed nanafibrous mat could turn out to be a suitable material for the wound dressing purpose.

摘要

在过去几年中，通过静电纺丝技术生产的纳米纤维垫显示出修复皮肤的潜在可行性。预计将植物提取物掺入这种结构可以为生物医学和伤口愈合应用提供更好的性能。本研究涉及制造基于 PVA 的纳米纤维垫，该垫装有一种名为Nigella sativa的传统植物的提取物，以增强抗菌、水分管理和伤口愈合特性。已经研究了开发的电纺纳米纤维的结构、粘合行为和水分管理特性。此外，对金黄色葡萄球菌（S. aureus）和大肠杆菌（E.coli）的抗菌活性还研究了上述垫子的细菌和伤口愈合活性。将黑种草提取物掺入 PVA 垫中表现出优异的抗菌活性，对两种细菌细胞形成 10.27 毫米和 7.8 毫米的抑菌圈。据观察，开发的垫子通过在皮肤上形成网络结构而迅速愈合伤口。SEM 分析表明形成了直径为 178.8 ± 16.9 nm 的纳米纤维，FTIR 证实了电纺纤维中存在黑种草提取物。此外，根据 AATCC 测试特性，开发的垫子被归类为渗水。因此，新开发的纳米纤维垫可能成为适合伤口敷料的材料。