Abstract Quantification of staling aldehydes from wort and beer requires sensitive instrumental analysis. In the brewing industry, this is for the most part accomplished by gas chromatography-mass spectrometry (GC-MS) using headspace solid-phase microextraction (HS-SPME) and on-fiber derivatization (OFD) with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBHA). With respect to lipid oxidation products (e.g., (E)-2-nonenal) and minor Strecker aldehydes (e.g., methional), the sensitivity of GC-EI-MS might not be sufficient to ensure reliable quantification and long-term stability regarding method performance. To overcome these problems, an elaborate gas chromatography-electron impact-tandem mass spectrometry (GC-EI-MS/MS) methodology was implemented. GC-EI-MS/MS improved sensitivity and reduced matrix effects resulting from overlapping PFBHA-oximes (PFBOs). It enabled reliable quantification of 15 selected aldehydes in different matrices (wort, beer) across a wide concentration range (0.01 − 1000 µg/L). Extensive method validation and long-term stability testing proved excellent method performance. Lastly, direct comparison of GC-EI-MS/MS and GC-EI-MS revealed that the quantification of low abundant aldehydes via GC-EI-MS/MS is more reproducible and that lower limits of quantification were achieved.

中文翻译：

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使用 HS-SPME 和纤维上衍生，通过 GC-EI-MS/MS 分析麦芽汁和啤酒中的某些陈旧醛

摘要 麦汁和啤酒中陈旧醛的定量需要灵敏的仪器分析。在酿造工业中，这大部分是通过气相色谱-质谱 (GC-MS) 使用顶空固相微萃取 (HS-SPME) 和使用 O-(2,3, 4,5,6-五氟苄基）羟胺（PFBHA）。对于脂质氧化产物（例如 (E)-2-壬烯醛）和少量 Strecker 醛（例如甲硫醛），GC-EI-MS 的灵敏度可能不足以确保有关方法的可靠定量和长期稳定性表现。为了克服这些问题，实施了精细的气相色谱-电子冲击-串联质谱 (GC-EI-MS/MS) 方法。GC-EI-MS/MS 提高了灵敏度并减少了由重叠 PFBHA 肟 (PFBO) 引起的基质效应。它能够在很宽的浓度范围 (0.01 - 1000 µg/L) 内对不同基质（麦芽汁、啤酒）中的 15 种选定醛进行可靠定量。广泛的方法验证和长期稳定性测试证明了出色的方法性能。最后，GC-EI-MS/MS 和 GC-EI-MS 的直接比较表明，通过 GC-EI-MS/MS 对低丰度醛的定量更具重现性，并且实现了较低的定量限。