ABSTRACT

Background and objectives

The aim of this study was to evaluate the role of T- and B-regulatory cells (Tregs and Bregs) in the pathogenesis of idiopathic granulomatous mastitis (IGM).

Methods

This study includes 47 patients with pathologically proven IGM (Group P) and 26 healthy subjects (Group C). The patients in Group P were divided into two groups according to whether their lesions were active (Group P_A, n: 21) or in remission (Group P_R, n: 26). By using flow-cytometry, the frequencies of CD3⁺CD4⁺CD45RA⁻Foxp3^high activated Tregs (aTregs), CD3⁺CD4⁺CD45RA⁻Foxp3^low non-suppressive Tregs, CD3⁺CD4⁺CD45RA⁺Foxp3^low resting Tregs (rTregs), CD3⁺CD4⁺CD25⁺Foxp3⁻ T-effector cells (Teff), total Tregs and Bregs were analyzed in all subjects.

Results

The frequency of the Teff cells was statistically higher in Group P when compared with Group C (p =.004). The Foxp3 expression of Treg cells and the frequency of non-suppressive Tregs in Group P were statistically lower than Group C (p =.032 and p =.02, respectively). In addition, Group P_R’s Foxp3 expressions were statistically lower than Group C (p =.027); Group P_R’s aTregs ratio was statistically lower than Group P_A (p =.021); and the non-suppressive Tregs ratio of Group P_R was lower than both Group P_A and Group C (p =.006 and p <.0001). No significant differences were seen Bregs and B cell subsets.

Conclusion

Significant changes in Foxp3 expression and Treg subsets were seen in patients with active IGM lesion and in remission. This study shows an intrinsic defect of Tregs in patients with IGM.

中文翻译：

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调节性 T 细胞和 B 细胞在特发性肉芽肿性乳腺炎发病机制中的作用

摘要

背景和目标

本研究的目的是评估 T 和 B 调节细胞（Tregs 和 Bregs）在特发性肉芽肿性乳腺炎 (IGM) 发病机制中的作用。

方法

该研究包括 47 名经病理证实的 IGM 患者（P 组）和 26 名健康受试者（C 组）。_{P组患者根据病变是否活跃（ P}组，n：21）或缓解（P组_， n：26）分为两组。通过使用流式细胞术，CD3 ⁺ CD4 ⁺ CD45RA ^- Foxp3^高活化 Tregs (aTregs)、CD3 ⁺ CD4 ⁺ CD45RA ^- Foxp3^低非抑制性 Tregs、CD3 ⁺ CD4 ⁺ CD45RA ⁺ Foxp3^低静息 Tregs (rTregs) 的频率， CD3 ⁺在所有受试者中分析了CD4 ⁺ CD25 ⁺ Foxp3 ^{- T 效应细胞 (Teff)、总 Treg 和 Breg。}

结果

与 C 组相比，P 组 Teff 细胞的频率在统计学上更高（p =.004）。P组Treg细胞的Foxp3表达和非抑制性Treg的频率在统计学上低于C组（分别为p =.032和p =.02）。此外， PR组的 Foxp3 表达在统计学上低于 C 组（_p = .027）；P _R组的 aTregs 比率在统计学上低于 P _A组（p =.021）；_{并且 P R}组的非抑制性 Tregs 比率低于 P _A组和 C 组 ( p =.006 和p<.0001)。Bregs和B细胞亚群没有显着差异。

结论

在具有活动性 IGM 病变和缓解的患者中观察到 Foxp3 表达和 Treg 亚群的显着变化。这项研究显示了 IGM 患者中 Treg 的内在缺陷。