Blending d‐lactic acid (d‐LA) with l‐lactic acid can significantly improve the thermostability of polylactic (PLA). Although microbial production of d‐LA under acidic conditions is beneficial for the reduction of production costs, the yield is low due to the acidic toxicity of the source strains. Herein, an Issatchenkia orientalis glycosylphosphatidylinositol‐anchored protein IoGas1, which is required for resistance to low pH and salt stress, was expressed in the YIP‐J‐C‐D‐A1 yeast strain. This strain was integrated with Escherichia coli d‐lactate dehydrogenase gene and several attenuated key pathway genes, including pyruvate decarboxylases (PDC1, PDC6), JEN1 (a monocarboxylate transporter), d‐lactate dehydrogenase1 (DLD1), l‐lactate cytochrome‐c oxidoreductase (CYB2) and alcohol dehydrogenase 1(ADH1).

中文翻译：

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通过在酿酒酵母中表达耐酸基因IoGAS1来提高低pH条件下D-乳酸的生产

将d-乳酸（d -LA）与l-乳酸混合可以显着提高聚乳酸（PLA）的热稳定性。尽管在酸性条件下微生物生产d ‐LA有助于降低生产成本，但由于源菌株的酸性毒性，收率较低。在此，YIP‐J‐C‐D‐A1酵母菌株表达了抗低pH和盐胁迫所需的东方伊萨香糖基糖基磷脂酰肌醇固定蛋白IoGas1。该菌株与大肠杆菌 d-乳酸脱氢酶基因和几个减毒的关键通路基因整合在一起，包括丙酮酸脱羧酶（PDC1）。，PDC6），JEN1（单羧酸转运蛋白），d-乳酸脱氢酶1（DLD1），l-乳酸细胞色素c氧化还原酶（CYB2）和酒精脱氢酶1（ADH1）。