1. Rapid DNA extraction is a critical barrier for routine and fieldable genetics tests for applications in conservation, such as illegal trafficking and fraudulent mislabelling.
2. Here, we develop a non‐thermal focused ultrasound extraction (FUSE) technique that creates a dense cloud of high‐pressure acoustic cavitation bubbles to disintegrate targeted tissues into an acellular debris, resulting in the rapid release of entrapped DNA.
3. In this work, we demonstrate the proof‐of‐concept of the FUSE technique by obtaining species identifiable sequences and shotgun sequencing reads from DNA extracted from Atlantic salmon Salmo salar tissues.
4. Having mitigated the key risks for this technique, we hypothesize future developments with this technology can be applied to accelerate and simplify DNA extraction from exceedingly difficult samples with complex tissue matrices (i.e. fibrous tissue and timber samples) in both laboratory and field settings.

中文翻译：

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聚焦超声提取（FUSE）用于从组织基质中快速提取DNA

1. 快速的DNA提取是常规和可现场进行的遗传学检测在保护中应用的关键障碍，例如非法贩运和欺诈性标签错误。
2. 在这里，我们开发了一种非热聚焦超声提取（FUSE）技术，该技术可产生密集的高压声空化气泡云，从而将目标组织分解成无细胞碎片，从而使捕获的DNA迅速释放。
3. 在这项工作中，我们通过从大西洋鲑鱼Salmo盐沼组织提取的DNA中获得物种可识别的序列和shot弹枪测序读数，来证明FUSE技术的概念验证。
4. 减轻了该技术的主要风险后，我们假设该技术的未来发展可用于加速和简化在实验室和现场环境中从具有复杂组织基质（即纤维组织和木材样品）的极其困难的样品中提取DNA。