• Plants use their innate immune system to defend against phytopathogens. As a part of this, pattern triggered‐immunity is activated via pattern recognition receptor (PRR) detection of pathogen‐associated molecular patterns (PAMPs). Although an increasing number of PAMPs have been identified, the PRRs for their recognition remain largely unknown.
• In the present study, we report a receptor‐like protein RE02 (Response to VmE02) in Nicotiana benthamiana, which mediates the perception of VmE02, a PAMP previously identified from the phytopathogenic fungus Valsa mali, using virus‐induced gene silencing (VIGS), co‐immunoprecipitation, pull‐down and microscale thermophoresis assays.
• We show that silencing of RE02 markedly attenuated VmE02‐triggred cell death and immune responses. RE02 specifically interacted with VmE02 in vivo and in vitro, and it displayed a high affinity for VmE02. Formation of a complex with the receptor‐like kinases SOBIR1 and BAK1 was essential for RE02 to perceive VmE02. Moreover, RE02‐silenced plants exhibited enhanced susceptibility to both the oomycete Phytophthora capsici and the fungus Sclerotinia sclerotiorum, while overexpression of RE02 increased plant resistance to these pathogens.
• Together, our results indicate that the PAMP VmE02 and the receptor‐like protein RE02 represent a new ligand–receptor pair in plant immunity, and that RE02 represents a promising target for engineering disease resistance.

中文翻译：

---

来自本塞姆氏烟草的受体样蛋白介导 VmE02 PAMP 触发的免疫

• 
  植物利用其先天免疫系统来防御植物病原体。作为其中的一部分，模式触发免疫是通过模式识别受体（PRR）检测病原体相关分子模式（PAMP）来激活的。尽管越来越多的 PAMP 已被识别，但识别它们的 PRR 仍然很大程度上未知。
• 
  在本研究中，我们报告了本塞姆氏烟草中的一种受体样蛋白 RE02（对 VmE02 的响应），它介导对 VmE02 的感知，VmE02 是先前使用病毒诱导的基因沉默（VIGS）从植物病原真菌Valsa mali中鉴定出的 PAMP，免疫共沉淀、下拉和微量热泳分析。
• 
  我们发现RE02的沉默显着减弱了 VmE02 触发的细胞死亡和免疫反应。 RE02在体内和体外与VmE02特异性相互作用，并且对VmE02表现出高亲和力。与受体样激酶 SOBIR1 和 BAK1 形成复合物对于 RE02 感知 VmE02 至关重要。此外， RE02沉默的植物表现出对卵菌辣椒疫霉和真菌核盘菌的易感性增强，而RE02的过度表达增加了植物对这些病原体的抗性。
• 
  总之，我们的结果表明，PAMP VmE02 和受体样蛋白 RE02 代表了植物免疫中的新配体-受体对，并且 RE02 代表了工程抗病性的有希望的靶标。