Calcium is a prerequisite factor for triggering the sperm activation in salmonids. The aim of this study was to test different concentrations of Ca²⁺ on sperm motility activation and fertility of rainbow trout (Oncorhynchus mykiss). Semen samples activated with calcium free activator (0.0 mM) and increasing concentrations of CaCl₂ (6.3, 7.9, 9.5 and 11 Mm) were evaluated by computer‐assisted sperm analysis (CASA). To assess fertility, eggs from three females were fertilized with semen and activated with each of the five solutions and incubated until four‐cell stage when the rate fertility and the symmetry of the first blastomeres were evaluated. The results show that eliminating calcium from the activator solution significantly reduced the motility rate and fertility in comparison with the other treatments, while increasing the CaCl₂ from 6.3 to 11 mM generated high sperm motility (78.5 ± 5.8–95.4 ± 4.0%) and fertility (85.0 ± 9.3–96.0 ± 6.51%) rates, and low symmetry rates in the first blastomeres (<20%). No significant differences were found between solutions for the latter two variables, suggesting that the calcium concentrations were in balance with the other components of the medium and within the requirements for the fertilization of rainbow trout.

中文翻译：

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不同钙浓度对虹鳟精子运动能力和受精能力的影响

钙是触发鲑鱼精子激活的前提因素。这项研究的目的是测试不同浓度的Ca ²⁺对虹鳟（Oncorhynchus mykiss）的精子活动性激活和生育能力。精液样品用无钙活化剂（0.0 mM）活化并增加了CaCl _2的浓度通过计算机辅助精子分析（CASA）评估（6.3、7.9、9.5和11 Mm）。为了评估生育力，对三名雌性卵进行精液受精，并用五种溶液中的每一种激活，并孵育至四细胞阶段，然后评估第一批卵裂球的繁殖率和对称性。结果表明，与其他处理相比，从活化剂溶液中消除钙显着降低了运动速率和生育力，同时增加了CaCl _2。从6.3到11 mM产生较高的精子运动力（78.5±5.8–95.4±4.0％）和受精率（85.0±9.3–96.0±6.51％）率，而第一卵裂球的对称率较低（<20％）。后两个变量的溶液之间没有发现显着差异，这表明钙浓度与培养基的其他成分保持平衡，并且处于虹鳟鱼受精的要求之内。