Indicator of Reduction In Soils (IRIS) technology is an important tool for identifying hydric soils, but it does not allow the user to monitor in real time. IRIS uses metal‐oxide coatings on a polyvinyl chloride surface that, under anaerobic conditions, are removed to varying degrees over a 4‐wk incubation period, during which time the user is not cognizant of the outcome. We document the viability of an alternative IRIS approach using clear‐IRIS tubes, made from cellulose acetate butyrate, that can be continuously monitored in situ with a Wi‐Fi–enabled video camera. This work shows that IRIS and clear‐IRIS tubes are statistically equivalent. Manganese‐oxide coated clear‐IRIS tubes correlated well with IRIS tubes (r = .79) and ferrous‐oxide had a high correlation (r = .97). A time‐series analysis showed that rain‐driven soil saturation induced IRIS metal‐oxide reduction and controlled the rate. Clear‐IRIS tubes enable remote sensing of metal‐oxide removal over time.

中文翻译：

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宏观和微观视觉成像工具研究土壤中的金属还原细菌

减少土壤中的指标（IRIS）技术是识别含水土壤的重要工具，但不允许用户实时监控。IRIS在聚氯乙烯表面上使用了金属氧化物涂层，在厌氧条件下，在4周的潜伏期中会不同程度地去除金属氧化物涂层，在此期间，用户不会意识到结果。我们记录了另一种使用透明IRIS管（由醋酸丁酸纤维素制成）的IRIS方法的可行性，该管可以用支持Wi-Fi的摄像机连续进行现场监测。这项工作表明，IRIS和clear-IRIS显像管在统计上是等效的。氧化锰涂层的透明IRIS管与IRIS管的相关性很好（r  = .79），而氧化亚铁具有很高的相关性（r = .97）。时间序列分析表明，雨水驱动的土壤饱和度导致IRIS金属氧化物减少并控制了速率。透明的IRIS管可实现随时间推移对金属氧化物去除的远程感测。