Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin‐derived peptide,Journal of Cellular Physiology

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Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin‐derived peptide
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-10-08 , DOI: 10.1002/jcp.30095
Sang Eun Park ₁ , Gyu-Bum Yeon ₂ , Hui-Gwan Goo ₁ , Dong Sik Seo ₁ , Ahmed A Dayem ₃ , Kyung Eun Lee ₄ , Hyun-Mee Park ₄ , Ssang-Goo Cho ₃ , Dae-Sung Kim _{2,

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Affiliation

Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long‐term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated. Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin‐derived peptide‐mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm‐coated PVDF scaffold (VNm–PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity. Under cardiac differentiation conditions, hESCs on the VNm–PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac‐related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm–PVDF scaffolds may be suitable for the long‐term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine.

中文翻译：

固定有玻连蛋白衍生肽的聚偏二氟乙烯支架上人类 ES 细胞的维持和分化

聚偏二氟乙烯（PVDF）具有生物相容性、易于制造且具有压电特性；它已用于许多生物医学应用，包括干细胞工程。然而，人类胚胎干细胞 (hESC) 的长期培养及其在 PVDF 上向心脏谱系的分化尚未得到研究。在此，通过静电纺丝制备了PVDF纳米级膜支架；玻连蛋白衍生的肽-贻贝粘附蛋白融合蛋白（VNm）被固定在支架上。在 VNm 涂层 PVDF 支架（VNm-PVDF 支架）上培养的 hESC 可以稳定扩增 10 代以上，同时保持多能性标记的表达和基因组完整性。在心脏分化条件下，与单独在 Matrigel 和 VNm 上培养的 hESC 相比，VNm-PVDF 支架上的 hESC 产生更多自发跳动的集落，并表现出心脏相关基因的上调。因此，VNm-PVDF支架可能适合hESC的长期培养及其向心肌细胞的分化，从而扩大其在再生医学中的应用。

更新日期：2020-10-08

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