Money spider dietary choice in pre‐ and post‐harvest cereal crops using metabarcoding,Ecological Entomology

当前位置： X-MOL 学术 › Ecol. Entomol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Money spider dietary choice in pre‐ and post‐harvest cereal crops using metabarcoding
Ecological Entomology ( IF 2.0 ) Pub Date : 2020-10-09 , DOI: 10.1111/een.12957
Jordan P. Cuff _{1,

2} , Lorna E. Drake ₁ , Maximillian P.T.G. Tercel ₁ , Jennifer E. Stockdale _{1,

3} , Pablo Orozco‐terWengel ₁ , James R. Bell ₂ , Ian P. Vaughan ₁ , Carsten T. Müller ₁ , William O.C. Symondson ₁

Affiliation

Money spiders (Linyphiidae) are an important component of conservation biological control in cereal crops, but they rely on alternative prey when pests are not abundant, such as between cropping cycles. To optimally benefit from these generalist predators, prey choice dynamics must first be understood. Money spiders and their locally available prey were collected from cereal crops 2 weeks pre‐ and post‐harvest. Spider gut DNA was amplified with two novel metabarcoding primer pairs designed for spider dietary analysis, and sequenced. The combined general and spider‐exclusion primers successfully identified prey from 15 families in the guts of the 46 linyphiid spiders screened, whilst avoiding amplification of Erigone spp. The primers show promise for application to the diets of other spider families such as Agelenidae and Pholcidae. Distinct invertebrate communities were identified pre‐ and post‐harvest, and changes in spider diet and, to a lesser extent, prey choice reflected this. Spiders were found to consume one another more than expected, indicating their propensity towards intraguild predation, but also consumed common pest families. Changes in spider prey choice may redress prey community changes to maintain a consistent dietary intake. Consistent provision of alternative prey via permanent refugia should be considered to sustain effective conservation biocontrol.

中文翻译：

使用元条形码技术在收获前和收获后谷物作物中的金钱蜘蛛饮食选择

金钱蜘蛛（Linyphiidae）是谷类作物保护性生物防治的重要组成部分，但当害虫不丰富时，例如在种植周期之间，它们依赖替代猎物。为了从这些多面手捕食者中获得最佳利益，必须首先了解猎物选择动态。金钱蜘蛛及其当地可用的猎物是在收获前和收获后 2 周从谷类作物中收集的。蜘蛛肠道 DNA 用两个专为蜘蛛饮食分析设计的新型元条形码引物对进行扩增，并进行测序。组合的一般引物和蜘蛛排除引物成功地从筛选的 46 只绦虫蜘蛛的肠道中识别了来自 15 个科的猎物，同时避免了 Erigone spp 的扩增。这些引物显示出适用于其他蜘蛛科（如阿格列尼达科和蜘蛛科）的饮食的希望。在收获前后确定了不同的无脊椎动物群落，蜘蛛饮食的变化以及在较小程度上猎物选择的变化反映了这一点。发现蜘蛛彼此之间的消耗量超过预期，表明它们倾向于在公会内捕食，但也消耗了常见的害虫家族。蜘蛛猎物选择的变化可能会纠正猎物社区的变化，以保持一致的饮食摄入量。应考虑通过永久性避难所持续提供替代猎物，以维持有效的保护生物防治。蜘蛛猎物选择的变化可能会纠正猎物社区的变化，以保持一致的饮食摄入量。应考虑通过永久性避难所持续提供替代猎物，以维持有效的保护生物防治。蜘蛛猎物选择的变化可能会纠正猎物群落的变化，以保持一致的饮食摄入量。应考虑通过永久性避难所持续提供替代猎物，以维持有效的保护生物防治。

更新日期：2020-10-09

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11