During the orthodontic tooth movement, cells in periodontal ligament could differentiate into osteoblasts to synthesize alveolar bone as well as affect the proliferation, migration and differentiation of mesenchymal stem cells, which also contribute to bone remodeling. However, the mechanism is still largely elusive. Here, we evaluated the expression of CREB at the tension site of mouse periodontal ligament under orthodontic mechanical strain and in the cyclic tension strain treated human periodontal ligament cells. Then, through gain and loss of function analysis, we revealed that CREB in PDLCs promotes SDF-1 and FGF2 secretion, which enhance the migration and osteoblastic differentiation of BMSCs. We further discovered that CREB transcriptionally activates FGF2 and SDF-1 expressions by binding to the promoter regions.In conclusion, this study confirms that CREB is an upregulated gene in periodontal ligament under orthodontic tension strain stimulation and plays an important role in regulating BMSCs’ physiological activity in orthodontic tension strain-induced bone formation.

在正畸牙齿运动过程中，牙周膜内的细胞可分化为成骨细胞，合成牙槽骨，并影响间充质干细胞的增殖、迁移和分化，也有助于骨重塑。然而，该机制在很大程度上仍然难以捉摸。在这里，我们评估了正畸机械应变下小鼠牙周膜张力部位和循环张力应变处理的人牙周膜细胞中 CREB ​​的表达。然后，通过功能得失分析，我们发现 PDLCs 中的 CREB ​​促进 SDF-1 和 FGF2 分泌，从而增强 BMSCs 的迁移和成骨细胞分化。我们进一步发现 CREB ​​通过与启动子区域结合来转录激活 FGF2 和 SDF-1 的表达。