In this study, a new molecularly imprinted material, MIP@UiO-66-NH₂, was synthesized with glutathione (GSH) as template and mesoporous metal organic framework (UiO-66-NH₂) as matrix. The molecularly imprinted polymer was modified on the surface and into the pores of the UiO-66-NH₂ by surface molecular imprinting method with thin polymer layer. Based on high specific surface area (1091.93 m² g⁻¹) and appropriate pore size (35 nm) of the ordered mesoporous UiO-66-NH₂, the adsorption capacity for GSH reached 94.43 mg g⁻¹, and the adsorption equilibrium could be achieved within 30 min. The adsorption isotherm data of MIP@UiO-66-NH₂ could be described well by Freundlich model and the kinetic data complied well with pseudo-second-order model. In addition, the MIP@UiO-66-NH₂ showed low adsorption capacity to GSH structural analogs (Q_L-cys = 6.51 mg g⁻¹), suggesting great selectivity for GSH recognition. Finally, the MIP@UiO-66-NH₂ was successfully applied for selective separation of GSH from BSA, skim milk and egg white tryptic digest.

本研究以谷胱甘肽（GSH）为模板，介孔金属有机骨架（UiO-66-NH ₂）为基质，合成了一种新型分子印迹材料MIP @ UiO-66-NH ₂。通过具有薄聚合物层的表面分子印迹方法，将分子印迹聚合物修饰在UiO-66-NH ₂的表面和孔中。基于有序介孔UiO-66-NH _2的高比表面积（1091.93 m ² g ^-1）和合适的孔径（35 nm），对GSH的吸附容量达到94.43 mg g ^-1，并且吸附平衡可以在30分钟内完成。MIP @ UiO-66-NH ₂的吸附等温线数据Freundlich模型可以很好地描述动力学数据，伪二阶模型可以很好地拟合动力学数据。此外，MIP @ UiO-66-NH ₂对GSH结构类似物的吸附能力较低（Q _L-cys = 6.51 mg g ^-1），表明对GSH的识别具有很高的选择性。最终，MIP @ UiO-66-NH ₂成功应用于从牛血清白蛋白，脱脂牛奶和蛋清胰蛋白酶消化物中选择性分离GSH。