Abstract Trace elements are present in minute amounts in the human body but contribute to its proper functioning, by participating in several biological processes. Imbalance of the concentrations of these elements can lead to the development of pathologies, including cancer. As such, the determination of trace element content in tumour tissues and its comparison with normal ones may be helpful for a better understanding of carcinogenesis. In this work, we address the collection and preparation of biological samples for Energy Dispersive X-Ray Fluorescence (EDXRF) analysis, and present a model for the quantification of trace elements, based on the external standard method of quantification. The model was used for the quantification of iron, copper, and zinc in a set of paired samples (normal and tumour tissues). The obtained results show the validity of the method and variations of the elemental concentrations in the different tissues.

中文翻译：

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外标法生物样品能量色散X射线荧光定量分析

摘要 微量元素在人体中微量存在，但通过参与多种生物过程有助于其正常运作。这些元素浓度的不平衡会导致病理的发展，包括癌症。因此，肿瘤组织中微量元素含量的测定及其与正常组织的比较可能有助于更好地了解致癌作用。在这项工作中，我们解决了用于能量色散 X 射线荧光 (EDXRF) 分析的生物样品的收集和制备，并基于外标量化方法提出了微量元素的量化模型。该模型用于定量一组配对样本（正常和肿瘤组织）中的铁、铜和锌。