Staphylococcus aureus and Staphylococcus epidermidis are among the most important bacterial species responsible for biofilm formation on indwelling medical devices, including orthopaedic implants. The increasing resistance to antimicrobials, partly attributed to the ability to form biofilms, is a challenge for the development of new antimicrobial agents. In this study, the cell-free supernatant obtained from sponge-associated Enterobacter strain 84.3 culture inhibited biofilm formation (>65%) and dissociated mature biofilm (>85%) formed by S. aureus and S. epidermidis strains. The culture supernatant was subjected to solvent partitioning and the aqueous extract presented a concentration-dependent antibiofilm activity for each strain with a minimum biofilm eradication concentration (MBEC) ranging from 16 to 256 μg/mL. The effect of the aqueous extract on mature S. aureus biofilm was analyzed by confocal scanning laser microscopy, showing a significant reduction of the biofilm layer as well as diminished interactions among the cells. This extract is not toxic for mammalian cells (L929 cell line). Studies targeting substances with antibiofilm activity gained significant attention in recent years due to difficult-to-treat biofilm infections. Here, sponge-associated Enterobacter 84.3 proved to be a source of substances capable of eradicating staphylococcal biofilm, with potential medical use in the future.

金黄色葡萄球菌和表皮葡萄球菌是导致留置医疗器械（包括骨科植入物）上生物膜形成的最重要细菌物种。抗菌剂耐药性的增加，部分归因于形成生物膜的能力，是开发新抗菌剂的挑战。在这项研究中，从海绵相关肠杆菌菌株 84.3 培养物中获得的无细胞上清液抑制了由金黄色葡萄球菌和表皮葡萄球菌形成的生物膜形成 (>65%) 和分离的成熟生物膜 (>85%)菌株。培养物上清液进行溶剂分配，水提取物对每个菌株都具有浓度依赖性的抗生物膜活性，最小生物膜根除浓度 (MBEC) 范围为 16 至 256 μg/mL。通过共聚焦扫描激光显微镜分析水提取物对成熟金黄色葡萄球菌生物膜的影响，显示生物膜层显着减少以及细胞之间的相互作用减弱。该提取物对哺乳动物细胞（L929 细胞系）无毒。由于难以治疗的生物膜感染，近年来针对具有抗生物膜活性的物质的研究受到了极大的关注。在这里，海绵相关肠杆菌 84.3 被证明是能够根除葡萄球菌生物膜的物质来源，在未来具有潜在的医疗用途。