In higher plants, floral signals are mainly collected and transduced to FLOWERING LOCUS T (FT) in Arabidopsis and its orthologues. The movement of FT from leaves to the shoot apical meristem (SAM) is partially mediated by FT-INTERACTING PROTEIN1 (FTIP1). Although the functions of OsFTIP1 in rice and DOFTIP1 in orchid in FT transport have also been investigated, the FTIP1 homologue in lotus (Nelumbo nucifera Gaertn.), a type of horticultural plant with high economic and cultural value, has not been isolated, and the mechanism of NnFT1 transport has not been explored. Here, we revealed that NnFTIP1 mediates the transport of NnFT1 in ectopic transgenic lines in Arabidopsis. Overexpression of NnFTIP1 in the ftip1-1 background rescued the late flowering phenotype of ftip1-1, indicating that NnFTIP1 has a conserved function as FTIP1. NnFTIP1 and NnFT1 share similar tissue expression patterns and subcellular localization. NnFTIP1 and NnFT1 interact both in vitro and in vivo. In addition, NnFTIP1 affects NnFT1 transport from leaves to the SAM. Furthermore, we found that NnUOF8, a MYB-like transcription factor, directly regulates the expression of NnFTIP1. Our results suggest that the functions of FTIP1 and FT are conserved during evolution in flowering plants.

中文翻译：

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莲花 NnFTIP1 和 NnFT1 调节拟南芥开花时间


在高等植物中，花信号主要被收集并转导至拟南芥及其直系同源物中的FLOWERING LOCUS T (FT)。 FT 从叶子到茎尖分生组织 (SAM) 的运动部分是由 FT 相互作用蛋白 1 (FTIP1) 介导的。虽然水稻中的 OsFTIP1 和兰花中的 DOFTIP1 在 FT 运输中的功能也已被研究，但在具有较高经济和文化价值的园艺植物荷花（Nelumbo nucifera Gaertn.）中 FTIP1 同源物尚未分离出来，并且NnFT1 运输机制尚未被探索。在这里，我们揭示了 NnFTIP1 介导拟南芥异位转基因系中 NnFT1 的转运。在ftip1-1背景中NnFTIP1的过表达挽救了ftip1-1的晚花表型，表明NnFTIP1具有与FTIP1一样的保守功能。 NnFTIP1 和 NnFT1 具有相似的组织表达模式和亚细胞定位。 NnFTIP1 和 NnFT1 在体外和体内均相互作用。此外，NnFTIP1 影响 NnFT1 从叶子到 SAM 的运输。此外，我们发现NnUOF8（一种MYB样转录因子）直接调节NnFTIP1的表达。我们的结果表明 FTIP1 和 FT 的功能在开花植物的进化过程中是保守的。