Neuropeptide Y(NPY) is known to play a pivotal role in various physiological functions including appetite and reproduction. While studies in mammals, fishes and reptiles suggest a temporal and evolutionary conserved role of NPY, the information in amphibian is scanty. We have investigated the reproductive phase related variations of NPY in the brain of Microhyla ornata (M. ornata), using immunohistochemistry and reverse transcription quantitative PCR (RT-qPCR). The highest expression of NPY peptide was observed in the preoptic area (Poa), nucleus infundibularis ventralis (NIV) and nucleus reticularis isthmi (NRIS) of M. ornata in breeding season compared to pre-breeding as well as post-breeding season. In parallel, highest mRNA levels of NPY were also observed in the breeding season in the middle region of brain that includes hypothalamus of M. ornata. Variation in the levels of NPY peptide and mRNA levels in the brain of M. ornata point towards seasonal control of appetite and reproduction.

已知神经肽Y（NPY）在各种食欲和生殖等生理功能中起着关键作用。尽管对哺乳动物，鱼类和爬行动物的研究表明，NPY具有暂时性和进化性的保守作用，但两栖动物中的信息却很少。我们已经使用免疫组织化学和逆转录定量PCR（RT-qPCR）研究了Microhyla ornata（M. ornata）大脑中与繁殖期相关的NPY变异。NPY肽的最高表达出现在M. ornata的视前区（Poa），漏斗腹侧核（NIV）和网状核峡部（NRIS）中。与繁殖前和繁殖后季节相比 同时，在繁殖季节的中部大脑中部（包括ornata M. ornata）的下丘脑中也观察到了最高的NPY mRNA水平。悬链念珠菌脑中NPY肽水平和mRNA水平的变化表明食欲和繁殖的季节性控制。