The aim of the present in vivo study was to evaluate the efficacy of a novel multicomponent mycotoxin detoxifying agent, as mineral premix consisting of modified zeolite, Bacillus spp., yeast cell wall and silymarin (MMDA; Patent Co, Misicevo, Serbia), in-feed to adsorb mycotoxins zearalenone (ZEN) and T-2, administered through feed in weaned pigs. In total, 112 post-weaning barrows and gilts (Danbred x Piétrain) from the 25th day of age (day 0 of the trial) until 66th day of age (end of trial) were allocated in four trial groups. Each group consisted of 28 pigs placed in seven pens (replicates) with four animals in each pen. Group G1 served as control group that received feed without any mycotoxins or MMDA; Group G2 received feed with spiked corn and wheat reaching contamination levels of 0.35 mg ZEN/kg and 0.5 mg T2/kg feed. Group G3 received contaminated feed (similar contamination levels with G2 group) with the additions of 1 g MMDA/kg, whereas G4 group received also contaminated feed (similar contamination levels with G2 group) with the addition of 3 g MMDA/kg. Growth performance (body weight, average daily gain, feed conversion ratio), as well as hematological and serum biochemical parameters, fecal scoring, gross pathology, and mycotoxins residue levels in liver, spleen, kidneys and muscle tissue were assessed. Results suggested that inclusion of MMDA in trial diets induced dose-dependent reduction of genital organs (sum of ovaries, cornu uteri and vagina-vestibule) relative weight and vulva size, which had been increased due to ZEN ingestion in G2 group. Additionally, in both groups that received MMDA, significantly reduced residue levels of ZEN in liver and kidney samples were observed, in comparison with G2 group. Significant residue levels reduction of T-2/HT-2 toxins was also observed in kidney samples of MMDA-fed groups when compared with G2 group. Taken together MMDA added to diets containing ZEN and T-2 toxin has a dose dependent affinity for ZEN and to a lower extend for T2 toxin, whereas such effect seems greater at inclusion level of 3 g MMDA/kg feed.

本体内研究的目的是评估新型多组分霉菌毒素解毒剂作为由改性沸石芽孢杆菌组成的矿物质预混合物的功效。spp。，酵母细胞壁和水飞蓟素（MMDA；塞尔维亚Co. Misicevo的Patent Co）的饲料，通过吸收断奶猪的饲料来吸收霉菌毒素玉米赤霉烯酮（ZEN）和T-2。从第25日龄（试验第0天）到第66日龄（试验结束），总共分为112个断奶后的公猪和后备母猪（Danbred xPiétrain）。每组由28只猪组成，每只猪放在7支（重复）中，每只有4只动物。G1组作为对照组，接受不含任何霉菌毒素或MMDA的饲料；G2组接受加标玉米和小麦的饲料，污染水平为0.35 mg ZEN / kg和0.5 mg T2 / kg饲料。G3组接受了污染的饲料（与G2组相似的污染水平），添加了1 g MMDA / kg，而G4组也接受了污染的饲料（与G2组相似的污染水平），添加了3 g MMDA / kg。评估生长性能（体重，平均日增重，饲料转化率）以及血液学和血清生化参数，粪便评分，总体病理以及肝，脾，肾和肌肉组织中的霉菌毒素残留水平。结果表明，在试验饮食中加入MMDA会导致生殖器官（卵巢，角膜子宫和阴道血管的总和）相对重量和外阴大小的剂量依赖性降低，而G2组由于ZEN摄入而增加了这种消瘦。此外，与G2组相比，在接受MMDA的两组中，均观察到肝和肾样品中ZEN的残留水平显着降低。与G2组相比，在MMDA喂养组的肾脏样本中还观察到T-2 / HT-2毒素的残留水平显着降低。总的来说，添加到含ZEN和T-2毒素的日粮中的MMDA对ZEN具有剂量依赖性，对T2毒素的亲和力较低，而在3 g MMDA / kg饲料中，这种作用似乎更大。